SERCA pump isoform expression in endothelium of veins and arteries: Every endothelium is not the same

被引：21

作者：

Khan, I

Sandhu, V

Misquitta, C

Grover, A ^{[1
]}

机构：

[1] McMaster Univ, Dept Med, Hamilton, ON L8N 3Z5, Canada

[2] Kuwait Univ, Fac Med, Dept Biochem, Kuwait, Kuwait

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2000年 / 203卷 / 1-2期

关键词：

coronary artery; coronary vein; aorta; vena cava; sarcoplasmic reticulum; endoplasmic reticulum; ATPase; calcium pump; smooth muscle; ischemia-reperfusion; signal transduction; transport; cardiovascular; cDNA; mRNA;

D O I：

10.1023/A:1007093516593

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Endothelium from rat aorta expresses sarco/endoplasmic reticulum Ca2+(SERCA) pump gene SERCA3 where as the smooth muscle expresses SERCA2. This has led to the postulate that vascular endothelium expresses SERCA3. To test this postulate, we examined the SERCA2 and SERCA3 mRNA expression in endothelium and smooth muscle dissected from coronary artery, coronary vein, aorta and vena cava of pig. Smooth muscle from all arteries and veins expressed only the SERCA2 mRNA. Endothelium from coronary artery, coronary vein and aorta expressed both SERCA2 and SERCA3 mRNA but the endothelium from vena cava did not express SERCA3 mRNA although it expressed SERCA2. These observations support the postulate that vascular endothelium expresses SERCA3 but the affirmation is equivocal because vena cava endothelium does not express SERCA3.

引用

页码：11 / 15

页数：5

共 31 条

[1] THE SARCO(ENDO)PLASMIC RETICULUM CA2+-ATPASE MESSENGER-RNA ISOFORM, SERCA-3, IS EXPRESSED IN ENDOTHELIAL AND EPITHELIAL-CELLS IN VARIOUS ORGANS [J].