Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa

被引：65

作者：

Costa, Siddhartha G. V. A. O. ^{[1
]}

Lepine, Francois ^{[2
]}

Milot, Sylvain ^{[2
]}

Deziel, Eric ^{[2
]}

Nitschke, Marcia ^{[3
]}

Contiero, Jonas ^{[1
]}

机构：

[1] Sao Paulo State Univ UNESP, Dept Biochem & Microbiol, Inst Biol Sci, BR-13506900 Rio Claro, SP, Brazil

[2] Univ Quebec, Inst Armand Frappier, INRS, Laval, PQ H7V 1B7, Canada

[3] Univ Sao Paulo, Dept Phys Chem, Inst Chem, BR-13560970 Sao Carlos, SP, Brazil

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 2009年 / 36卷 / 08期

基金：

巴西圣保罗研究基金会;

关键词：

Cassava wastewater; Glycerol; Polyhydroxyalkanoates; Pseudomonas aeruginosa; Rhamnolipids; Waste cooking oil; BIOSURFACTANT PRODUCTION; STRAIN; 57RP; OIL; POLYESTERS; CULTURE; LBI;

D O I：

10.1007/s10295-009-0590-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glycerol, cassava wastewater (CW), waste cooking oil and CW with waste frying oils were evaluated as alternative low-cost carbon substrates for the production of rhamnolipids and polyhydroxyalkanoates (PHAs) by various Pseudomonas aeruginosa strains. The polymers and surfactants produced were characterized by gas chromatography-mass spectrophotometry (MS) and by high-performance liquid chromatography-MS, and their composition was found to vary with the carbon source and the strain used in the fermentation. The best overall production of rhamnolipids and PHAs was obtained with CW with frying oil as the carbon source, with PHA production corresponding to 39% of the cell dry weight and rhamnolipid production being 660 mg l(-1). Under these conditions, the surface tension of the culture decreased to 30 mN m(-1), and the critical micelle concentration was 26.5 mg l(-1). It would appear that CW with frying oil has the highest potential as an alternative substrate, and its use may contribute to a reduction in the overall environmental impact generated by discarding such residues.

引用

页码：1063 / 1072

页数：10

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