Organization of Circadian Behavior Relies on Glycinergic Transmission

被引：63

作者：

Frenkel, Lia ^{[1
]}

Muraro, Nara I. ^{[6
]}

Beltran Gonzalez, Andrea N. ^{[2
]}

Marcora, Maria S. ^{[3
]}

Bernabo, Guillermo ^{[1
]}

Hermann-Luibl, Christiane ^{[4
]}

Romero, Juan I. ^{[1
]}

Helfrich-Forster, Charlotte ^{[4
]}

Castano, Eduardo M. ^{[3
]}

Marino-Busjle, Cristina ^{[5
]}

Calvo, Daniel J. ^{[2
]}

Fernanda Ceriani, M. ^{[1
]}

机构：

[1] Consejo Nacl Invest Cient & Tecn, IIBBA, FIL, Lab Genet Comportamiento, 1405BWE, Buenos Aires, DF, Argentina

[2] Consejo Nacl Invest Cient & Tecn, INGEBI, Lab Neurobiol Celular & Mol, C1428ADN, Buenos Aires, DF, Argentina

[3] Consejo Nacl Invest Cient & Tecn, IIBBA, FIL, Lab Amiloidosis & Neurodegenerac, 1405BWE, Buenos Aires, DF, Argentina

[4] Univ Wurzburg, Theodor Boveri Inst, Dept Neurobiol & Genet, Bioctr, D-97070 Wurzburg, Germany

[5] Consejo Nacl Invest Cient & Tecn, IIBBA, FIL, Lab Bioinformat Estruct, 1405BWE, Buenos Aires, DF, Argentina

[6] Consejo Nacl Invest Cient & Tecn, MPSP, Biomed Res Inst Buenos Aires IBioBA, C1425FQD, Buenos Aires, DF, Argentina

来源：

CELL REPORTS | 2017年 / 19卷 / 01期

关键词：

DROSOPHILA-MELANOGASTER; CLOCK NEURONS; PACEMAKER NEURONS; SUPRACHIASMATIC NUCLEUS; LOCOMOTOR-ACTIVITY; ENDOGENOUS CLOCK; LATERAL NEURONS; NEUROPEPTIDE F; PDF NEURONS; RECEPTOR;

D O I：

10.1016/j.celrep.2017.03.034

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The small ventral lateral neurons (sLNvs) constitute a central circadian pacemaker in the Drosophila brain. They organize daily locomotor activity, partly through the release of the neuropeptide pigment-dispersing factor (PDF), coordinating the action of the remaining clusters required for network synchronization. Despite extensive efforts, the basic principles underlying communication among circadian clusters remain obscure. We identified classical neurotransmitters released by sLNvs through disruption of specific transporters. Adult-specific RNAi-mediated downregulation of the glycine transporter or impairment of glycine synthesis in LNv neurons increased period length by nearly an hour without affecting rhythmicity of locomotor activity. Electrophysiological recordings showed that glycine reduces spiking frequency in circadian neurons. Interestingly, downregulation of glycine receptor subunits in specific sLNv targets impaired rhythmicity, revealing involvement of glycine in information processing within the network. These data identify glycinergic inhibition of specific targets as a cue that contributes to the synchronization of the circadian network.

引用

页码：72 / 85

页数：14

共 70 条

[1] A GABAergic mechanism is necessary for coupling dissociable ventral and dorsal regional oscillators within the circadian clock [J].