Diagnosis of neuroschistosomiasis by antibody specificity index and semi-quantitative real-time PCR from cerebrospinal fluid and serum

被引：18

作者：

Haerter, Georg ^{[1
,2
]}

Frickmann, Hagen ^{[3
,4
]}

Zenk, Sebastian ^{[5
]}

Wichmann, Dominic ^{[6
]}

Ammann, Bettina ^{[7
]}

Kern, Peter ^{[1
,2
]}

Fleischer, Bernhard ^{[8
]}

Tannich, Egbert ^{[8
]}

Poppert, Sven ^{[8
]}

机构：

[1] Univ Hosp Ulm, Dept Infect Dis & Clin Immunol, Comprehens Infect Dis Ctr Ulm, Ulm, Germany

[2] Univ Hosp Ulm, Clin Internal Med 3, Ulm, Germany

[3] German Armed Forces Hosp Hamburg, Bernhard Nocht Inst, Dept Trop Med, Hamburg, Germany

[4] Univ Hosp Rostock, Inst Microbiol Virol & Hyg, Rostock, Germany

[5] Univ Hosp Ulm, Inst Med Microbiol & Hyg, Ulm, Germany

[6] Univ Hosp Eppendorf, Dept Intens Care Med, Hamburg, Germany

[7] Univ Hosp Ulm, Dept Diagnost & Intervent Radiol, Ulm, Germany

[8] Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 2014年 / 63卷

关键词：

SPINAL-CORD SCHISTOSOMIASIS; LINKED-IMMUNOSORBENT-ASSAY; CEREBRAL SCHISTOSOMIASIS; NEUROLOGICAL COMPLICATIONS; MANSONI MYELORADICULOPATHY; INFECTION; PATHOGENESIS; HAEMATOBIUM;

D O I：

10.1099/jmm.0.066142-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe the case of a 16-year-old German male expatriate from Ghana who presented with obstipation, dysuria, dysaesthesia of the gluteal region and the lower limbs, bilateral plantar hypaesthesia and paraesthesia without pareses. A serum cerebrospinal fluid (CSF) Schistosoma spp. specific antibody specificity index of 3.1 was considered highly suggestive of intrathecal synthesis of anti-Schistosoma spp. specific antibodies, although standardization of this procedure has not previously been described. Diagnosis was confirmed by detection of Schistosoma DNA in CSF by semi-quantitative real-time PCR at 100-fold concentration compared with serum. Accordingly the two diagnostic procedures, which have not previously been applied for routine diagnosis, appear to be useful for the diagnosis of neuroschistosomiasis. Clinical symptoms resolved following anthelmintic and anti-inflammatory therapy.

引用

页码：309 / 312

页数：4

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