The effect of photodynamic treatment combined with antibiotic action or host defence mechanisms on Staphylococcus aureus biofilms

被引:144
作者
Di Poto, Antonella
Sbarra, Maria S.
Provenza, Giulio
Visai, Livia [2 ]
Speziale, Pietro [1 ]
机构
[1] Univ Pavia, Fac Med & Surg, Dept Biochem, I-27100 Pavia, Italy
[2] Univ Pavia, Ctr Tissue Engn, I-27100 Pavia, Italy
关键词
Staphylococcus; Biofilm; Photodynamic treatment; Vancomycin; Phagocytosis; POLY-N-ACETYLGLUCOSAMINE; EPIDERMIDIS; POLYSACCHARIDE; EXPRESSION; PREVENTION; ADHERENCE; PROTECTS; ADHESION; THERAPY; RELEASE;
D O I
10.1016/j.biomaterials.2009.02.038
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Staphylococcus aureus is one of the most important etiological agents of infections associated with medical devices. This is in part due to the ability of the organism to form biofilm, which provides a microenvironment that protects from attack by the host's immune system and by antibiotics. In this study we examined the structure of polysaccharide intercellular adhesin (PIA)-dependent or protein-based S. aureus biofilms. We defined new strategies aimed at treatment of mature established biofilms using photodynamic treatment (PDT) combined with chemotherapy or phagocytosis. Significant inactivation of bacteria was observed when structurally distinct biofilms were exposed to the cationic porphyrin, tetra-substituted N-methyl-pyridyl-porphine (TMP), and simultaneously to visible light. Moreover, PDT-treated biofilms exposed to vancomycin or subjected to the phagocytic action of whole blood resulted in their almost complete eradication. The drastic reduction in staphylococcal survival and the disruption of biofilms were confirmed by confocal laser scanning microscopy and scanning electron microscopy. The results suggest that PDT combined with vancomycin and the host defences may be a useful approach for the inactivation of staphylococcal biofilms adhering to medical implant surfaces. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3158 / 3166
页数:9
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