Characterization of the glycosyltransferase activity of DesVII:: Analysis of and implications for the biosynthesis of macrolide antibiotics

被引：72
作者：
Borisova, SA
Zhao, LS
Melancon, CE
Kao, CL
Liu, HW [1 ]
机构：
[1] Univ Texas, Coll Pharm, Div Med Chem, Austin, TX 78712 USA
[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 21期
关键词：
D O I：
10.1021/ja049967j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In vitro catalytic activity of DesVII, the glycosyltransferase involved in the biosynthesis of methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described. This is the first report of demonstrated in vitro activity of a glycosyltransferase involved in the biosynthesis of macrolide antibiotics. DesVII is unique among glycosyltransferases in that it requires an additional protein component, DesVIII, as well as basic pH for its full activity. Copyright © 2004 American Chemical Society.
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收藏
页码：6534 / 6535
页数：2
相关论文
共 18 条
[1]   Substrate specificity of NovM: Implications for novobiocin biosynthesis and glycorandomization [J].
Albermann, C ;
Soriano, A ;
Jiang, JQ ;
Vollmer, H ;
Biggins, JB ;
Barton, WA ;
Lesniak, J ;
Nikolov, DB ;
Thorson, JS .
ORGANIC LETTERS, 2003, 5 (06) :933-936
[2]   PLASMID CLONING VECTORS FOR THE CONJUGAL TRANSFER OF DNA FROM ESCHERICHIA-COLI TO STREPTOMYCES SPP [J].
BIERMAN, M ;
LOGAN, R ;
OBRIEN, K ;
SENO, ET ;
RAO, RN ;
SCHONER, BE .
GENE, 1992, 116 (01) :43-49
[3]   MACROLIDE BIOSYNTHESIS .7. INCORPORATION OF POLYKETIDE CHAIN ELONGATION INTERMEDIATES INTO METHYMYCIN [J].
CANE, DE ;
LAMBALOT, RH ;
PRABHAKARAN, PC ;
OTT, WR .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (02) :522-526
[4]   Formation of unusual sugars: Mechanistic studies and biosynthetic applications [J].
He, XMM ;
Liu, HW .
ANNUAL REVIEW OF BIOCHEMISTRY, 2002, 71 :701-754
[5]   Mechanisms and pathways from recent deoxysugar biosynthesis research [J].
Johnson, DA ;
Liu, HW .
CURRENT OPINION IN CHEMICAL BIOLOGY, 1998, 2 (05) :642-649
[6]   Glycosides in medicine:: "The role of glycosidic residue in biological activity" [J].
Kren, V ;
Martínková, L .
CURRENT MEDICINAL CHEMISTRY, 2001, 8 (11) :1303-1328
[7]   PATHWAYS AND MECHANISMS IN THE BIOGENESIS OF NOVEL DEOXYSUGARS BY BACTERIA [J].
LIU, HW ;
THORSON, JS .
ANNUAL REVIEW OF MICROBIOLOGY, 1994, 48 :223-256
[8]   Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: Novel glycopeptides [J].
Losey, HC ;
Peczuh, MW ;
Chen, Z ;
Eggert, US ;
Dong, SD ;
Pelczer, I ;
Kahne, D ;
Walsh, CT .
BIOCHEMISTRY, 2001, 40 (15) :4745-4755
[9]   STOFFWECHSELPRODUKTE VON ACTINOMYCETEN .31. UBER DIE KONSTITUTION DES NARBOMYCINS [J].
PRELOG, V ;
TALBOT, G ;
ZAMOJSKI, A ;
GOLD, AM .
HELVETICA CHIMICA ACTA, 1962, 45 (01) :4-&
[10]   Glycosylation of macrolide antibiotics -: Purification and kinetic studies of a macrolide glycosyltransferase from Streptomyces antibioticus [J].
Quirós, LM ;
Carbajo, RJ ;
Braña, AF ;
Salas, JA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (16) :11713-11720
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