Characterization of the glycosyltransferase activity of DesVII:: Analysis of and implications for the biosynthesis of macrolide antibiotics

被引：72
作者：
Borisova, SA
Zhao, LS
Melancon, CE
Kao, CL
Liu, HW [1 ]
机构：
[1] Univ Texas, Coll Pharm, Div Med Chem, Austin, TX 78712 USA
[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 21期
关键词：
D O I：
10.1021/ja049967j
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In vitro catalytic activity of DesVII, the glycosyltransferase involved in the biosynthesis of methymycin, neomethymycin, narbomycin, and pikromycin in Streptomyces venezuelae, is described. This is the first report of demonstrated in vitro activity of a glycosyltransferase involved in the biosynthesis of macrolide antibiotics. DesVII is unique among glycosyltransferases in that it requires an additional protein component, DesVIII, as well as basic pH for its full activity. Copyright © 2004 American Chemical Society.
引用
收藏
页码：6534 / 6535
页数：2
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