A Dynamic Interface between Vacuoles and Mitochondria in Yeast

被引：289
作者：
Elbaz-Alon, Yael [1 ]
Rosenfeld-Gur, Eden [2 ]
Shinder, Vera [3 ]
Futerman, Anthony H. [2 ]
Geiger, Tamar [4 ]
Schuldiner, Maya [1 ]
机构：
[1] Weizmann Inst Sci, Dept Mol Genet, IL-7610001 Rehovot, Israel
[2] Weizmann Inst Sci, Dept Biol Chem, IL-7610001 Rehovot, Israel
[3] Weizmann Inst Sci, Electron Microscopy Unit, IL-7610001 Rehovot, Israel
[4] Tel Aviv Univ, Sack ler Fac Med, Dept Human Mol Genet & Biochem, IL-6997801 Tel Aviv, Israel
来源：
DEVELOPMENTAL CELL | 2014年 / 30卷 / 01期
基金：
以色列科学基金会; 欧洲研究理事会;
关键词：
SACCHAROMYCES-CEREVISIAE; MEMBRANE CONTACT; LIPID EXCHANGE; ER; PROTEINS; DELETION; PURIFICATION; REVEALS; DISEASE; COMPLEX;
D O I：
10.1016/j.devcel.2014.06.007
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure (ERMES) was identified as an important yet nonessential contact for such transport. Using a high-content screen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (vacuole and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other, and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk.
引用
收藏
页码：95 / 102
页数：8
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