Unanimous Model for Describing the Fast Bioluminescence Kinetics of Ca2+-regulated Photoproteins of Different Organisms

被引：10

作者：

Eremeeva, Elena V. ^{[1
]}

Bartsev, Sergey I. ^{[2
]}

van Berkel, Willem J. H. ^{[3
]}

Vysotski, Eugene S. ^{[1
]}

机构：

[1] Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, Photobiol Lab, Krasnoyarsk, Russia

[2] Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, Theoret Biophys Lab, Krasnoyarsk, Russia

[3] Wageningen Univ & Res, Biochem Lab, Wageningen, Netherlands

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 2017年 / 93卷 / 02期

关键词：

GREEN-FLUORESCENT PROTEIN; AEQUORIN BIOLUMINESCENCE; SEQUENCE-ANALYSIS; CRYSTAL-STRUCTURE; RECOMBINANT OBELIN; MNEMIOPSIS-LEIDYI; LIGHT-EMISSION; APO-AEQUORIN; CALCIUM; EXPRESSION;

D O I：

10.1111/php.12664

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Upon binding their metal ion cofactors, Ca2+-regulated photoproteins display a rapid increase of light signal, which reaches its peak within milliseconds. In the present study, we investigate bioluminescence kinetics of the entire photoprotein family. All five recombinant hydromedusan Ca2+-regulated photoproteinsaequorin from Aequorea victoria, clytin from Clytia gregaria, mitrocomin from Mitrocoma cellularia and obelins from Obelia longissima and Obelia geniculatademonstrate the same bioluminescent kinetics pattern. Based on these findings, for the first time we propose a unanimous kinetic model describing the bioluminescence mechanism of Ca2+-regulated photoproteins.

引用

页码：495 / 502

页数：8

共 13 条

[1] Characterization of hydromedusan Ca2+-regulated photoproteins as a tool for measurement of Ca2+concentration
Malikova, Natalia P.
Burakova, Ludmila P.
Markova, Svetlana V.
Vysotski, Eugene S.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2014, 406 (23) : 5715 - 5726
[2] Exploring Bioluminescence Function of the Ca2+-regulated Photoproteins with Site-directed Mutagenesis
Eremeeva, Elena V.
Vysotski, Eugene S.
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2019, 95 (01) : 8 - 23
[3] Specific Activities of Hydromedusan Ca2+-Regulated Photoproteins
Malikova, Natalia P.
Eremeeva, Elena, V
Gulnov, Dmitry, V
Natashin, Pavel, V
Nemtseva, Elena, V
Vysotski, Eugene S.
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 2022, 98 (01) : 276 - 284
[4] Ca2+-Regulated Photoproteins: Effective Immunoassay Reporters
Frank, Ludmila A.
SENSORS, 2010, 10 (12) : 11287 - 11300
[5] Bioluminescent and biochemical properties of Cys-free Ca2+-regulated photoproteins obelin and aequorin
Eremeeva, Elena V.
Vysotski, Eugene S.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2017, 174 : 97 - 105
[6] Recombinant Ca2+-regulated photoproteins of ctenophores: current knowledge and application prospects
Burakova, Lyudmila P.
Vysotski, Eugene S.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 103 (15) : 5929 - 5946
[7] Characterization of hydromedusan Ca2+-regulated photoproteins as a tool for measurement of Ca2+concentration
Natalia P. Malikova
Ludmila P. Burakova
Svetlana V. Markova
Eugene S. Vysotski
Analytical and Bioanalytical Chemistry, 2014, 406 : 5715 - 5726
[8] Structures of the Ca2+-regulated photoprotein obelin Y138F mutant before and after bioluminescence support the catalytic function of a water molecule in the reaction
Natashin, Pavel V.
Ding, Wei
Eremeeva, Elena V.
Markova, Svetlana V.
Lee, John
Vysotski, Eugene S.
Liu, Zhi-Jie
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2014, 70 : 720 - 732
[9] Ca2+-regulated photoproteins: structure, bioluminescent reaction mechanism, engineering, and application
Vysotski, E. S.
Markova, S., V
Frank, L. A.
Malikova, N. P.
Stepanyuk, G. A.
Burakova, L. P.
Eremeeva, E., V
Golz, S.
Lee, J.
LUMINESCENCE, 2010, 25 (02) : 212 - 212
[10] Role of certain amino acid residues of the coelenterazine-binding cavity in bioluminescence of light-sensitive Ca2+-regulated photoprotein berovin
Burakova, Ludmila P.
Stepanyuk, Galina A.
Eremeeva, Elena V.
Vysotski, Eugene S.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2016, 15 (05) : 691 - 704

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