Diversity in the degree of sulfation and chain length of the glycosaminoglycan moiety of urinary trypsin inhibitor isomers

被引：12
作者：
Kakizaki, Ikuko
Takahashi, Ryouki
Ibori, Nobuyuki
Kojima, Kaoru
Takahashi, Teruno
Yamaguchi, Masanori
Kon, Atushi
Takagaki, Keiichi
机构：
[1] Hirosaki Univ, Sch Med, Dept Biochem, Hirosaki, Aomori 0368562, Japan
[2] Wakamoto Pharmaceut Co Ltd, Kanagawa 2580018, Japan
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2007年 / 1770卷 / 02期
关键词：
urinary trypsin inhibitor; glycosaminoglycan; chondroitin sulfate;
D O I：
10.1016/j.bbagen.2006.09.026
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Five isomers with different electric charge were fractionated from human urinary trypsin inhibitor (UTI) by anion exchange HPLC. Intact low-sulfated chondroitin 4-sulfate chains from the isomers were analyzed by HPLC and mass spectrometry. Unsaturated disaccharide composition analysis of the chondroitin sulfate chain revealed that the five isomers differ in the numbers of 4-sulfated disaccharide units. Intriguingly, we detected the presence of multiple novel isomers with different numbers of non-sulfated disaccharide units even in the same charge isomer fraction. Our results demonstrate that UTI can vary in terms of both the degree Of sulfation and the length of the low-sulfated chondroitin 4-sulfate chain. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：171 / 177
页数：7
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