Crystal structure of the ribosome recycling factor from Escherichia coli

被引：79

作者：

Kim, KK ^{[1
]}

Min, K

Suh, SW

机构：

[1] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, Chinju 660701, South Korea

[2] Seoul Natl Univ, Coll Nat Sci, Dept Chem, Seoul 151742, South Korea

来源：

EMBO JOURNAL | 2000年 / 19卷 / 10期

关键词：

crystal structure; protein synthesis; ribosome recycling factor; translation; tRNA;

D O I：

10.1093/emboj/19.10.2362

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have determined the crystal structure of the Escherichia coli ribosome recycling factor (RRF), which catalyzes the disassembly of the termination complex in protein synthesis. The L-shaped molecule consists of two domains: a triple-stranded antiparallel coiled-coil and an alp domain. The coil domain has a cylindrical shape and negatively charged surface, which are reminiscent of the anticodon arm of tRNA and domain IV of elongation factor EF-G. We suggest that RRF binds to the ribosomal A-site through its coil domain, which is a tRNA mimic. The relative position of the two domains is changed about an axis along the hydrophobic cleft in the hinge where the alkyl chain of a detergent molecule is bound. The tRNA mimicry and the domain movement observed in RRF provide a structural basis for understanding the role of RRF in protein synthesis.

引用

页码：2362 / 2370

页数：9

共 48 条

[1] Direct visualization of A-, P-, and E-site transfer RNAs in the Escherichia coli ribosome [J].

Agrawal, RK ;

Penczek, P ;

Grassucci, RA ;

Li, YH ;

Leith, A ;

Nierhaus, KH ;

Frank, J .

SCIENCE, 1996, 271 (5251) :1000-1002

[2] Visualization of elongation factor G on the Escherichia coli 70S ribosome:: The mechanism of translocation [J].

Agrawal, RK ;

Penczek, P ;

Grassucci, RA ;

Frank, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (11) :6134-6138

[3] EF-G-dependent GTP hydrolysis induces translocation accompanied by large conformational changes in the 70S ribosome [J].

Agrawal, RK ;

Heagle, AB ;

Penczek, P ;

Grassucci, RA ;

Frank, J .

NATURE STRUCTURAL BIOLOGY, 1999, 6 (07) :643-647

[4] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[5] Placement of protein and RNA structures into a 5 Å-resolution map of the 50S ribosomal subunit [J].

Ban, N ;

Nissen, P ;

Hansen, J ;

Capel, M ;

Moore, PB ;

Steitz, TA .

NATURE, 1999, 400 (6747) :841-847

[6] Initiation factors of protein biosynthesis in bacteria and their structural relationship to elongation and termination factors [J].

Brock, S ;

Szkaradkiewicz, K ;

Sprinzl, M .

MOLECULAR MICROBIOLOGY, 1998, 29 (02) :409-417

[7] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[8] STRUCTURE OF INFLUENZA HEMAGGLUTININ AT THE PH OF MEMBRANE-FUSION [J].

BULLOUGH, PA ;

HUGHSON, FM ;

SKEHEL, JJ ;

WILEY, DC .

NATURE, 1994, 371 (6492) :37-43

[9] X-ray crystal structures of 70S ribosome functional complexes [J].

Cate, JH ;

Yusupov, MM ;

Yusupova, GZ ;

Earnest, TN ;

Noller, HF .

SCIENCE, 1999, 285 (5436) :2095-2104

[10] VERIFICATION OF PROTEIN STRUCTURES - PATTERNS OF NONBONDED ATOMIC INTERACTIONS [J].

COLOVOS, C ;

YEATES, TO .

PROTEIN SCIENCE, 1993, 2 (09) :1511-1519

← 1 2 3 4 5 →