Evolution of Carbapenem-Resistant Serotype K1 Hypervirulent Klebsiella pneumoniae by Acquisition of blaVIM-1-Bearing Plasmid

被引：25
作者：
Dong, Ning [1 ,2 ]
Sun, Qiaoling [3 ]
Huang, Yonglu [3 ]
Shu, Lingbin [3 ]
Ye, Lianwei [1 ,2 ]
Zhang, Rong [3 ]
Chen, Sheng [1 ,2 ]
机构：
[1] Hong Kong PolyU Shen Zhen Res Inst, Food Safety & Technol Res Ctr, Shenzhen Key Lab Food Biol Safety Control, Shenzhen, Peoples R China
[2] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, State Key Lab Chirosci, Hung Hom,Kowloon, Hong Kong, Peoples R China
[3] Zhejiang Univ, Sch Med, Affiliated Hosp 2, Dept Clin Lab, Hangzhou, Zhejiang, Peoples R China
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2019年 / 63卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Klebsiella pneumoniae; VIM; carbapenem resistance; hypervirulence;
D O I：
10.1128/AAC.01056-19
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We report the identification of a carbapenem-resistant, hypervirulent Klebsiella pneumoniae (hvKp) strain which produced the carbapenemase VIM-1. Genomic analysis showed that the strain belonged to sequence type ST23 and serotype K1, a major hvKp clone, and harbored three resistance-encoding plasmids. Among them, a bla(VIM-1)-bearing plasmid was found to possess a mosaic structure presumably generated by multiple gene mobilization events. This finding indicates that hvKp actively acquires mobile resistance-encoding elements, facilitating simultaneous expression of hypervirulence and carbapenem-resistance.
引用
收藏
页数：5
相关论文
共 18 条
  • [1] Convergence of carbapenem-resistance and hypervirulence in Klebsiella pneumoniae
    Chen, Liang
    Kreiswirth, Barry N.
    [J]. LANCET INFECTIOUS DISEASES, 2018, 18 (01) : 2 - 3
  • [2] Clinical and Laboratory Standards Institute, 2016, M100S26 CLSI
  • [3] Primary osteomyelitis caused by an NDM-1-producing K. pneumoniae strain of the highly virulent sequence type 23
    Compain, Fabrice
    Vandenberghe, Alexis
    Gominet, Marie
    Genel, Nathalie
    Lebeaux, David
    Ramahefasolo, Astrid
    Podglajen, Isabelle
    Decre, Dominique
    [J]. EMERGING MICROBES & INFECTIONS, 2017, 6 : 1 - 3
  • [4] A Novel IncA/C1 Group Conjugative Plasmid, Encoding VIM-1 Metallo-Beta-Lactamase, Mediates the Acquisition of Carbapenem Resistance in ST104 Klebsiella pneumoniae Isolates from Neonates in the Intensive Care Unit of V. Monaldi Hospital in Naples
    Esposito, Eliana P.
    Gaiarsa, Stefano
    Del Franco, Mariateresa
    Crivaro, Valeria
    Bernardo, Mariano
    Cuccurullo, Susanna
    Pennino, Francesca
    Triassi, Maria
    Marone, Piero
    Sassera, Davide
    Zarrilli, Raffaele
    [J]. FRONTIERS IN MICROBIOLOGY, 2017, 8
  • [5] A novel IncA plasmid carrying blaVIM-1 in a Kluyvera cryocrescens strain
    Gaibani, Paolo
    Ambretti, Simone
    Scaltriti, Erika
    Cordovana, Miriam
    Berlingeri, Andrea
    Pongolini, Stefano
    Landini, Maria Paola
    Re, Maria Carla
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2018, 73 (11) : 3206 - 3208
  • [6] Mobilization of a Tn402-Like Class 1 Integron with a Novel Cassette Array via Flanking Miniature Inverted-Repeat Transposable Element-Like Structures
    Gillings, Michael R.
    Labbate, Maurizio
    Sajjad, Ammara
    Giguere, Nellie J.
    Holley, Marita P.
    Stokes, H. W.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (18) : 6002 - 6004
  • [7] Gu DX, 2018, LANCET INFECT DIS, V18, P37, DOI [10.1016/S1473-3099(17)30489-9, 10.1016/s1473-3099(17)30489-9]
  • [8] Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations
    Lam, Margaret M. C.
    Wick, Ryan R.
    Wyres, Kelly L.
    Gorrie, Claire L.
    Judd, Louise M.
    Jenney, Adam W. J.
    Brisse, Sylvain
    Holt, Kathryn E.
    [J]. MICROBIAL GENOMICS, 2018, 4 (09):
  • [9] Vaccination with Klebsiella pneumoniae-derived extracellular vesicles protects against bacteria-induced lethality via both humoral and cellular immunity
    Lee, Won-Hee
    Choi, Hyun-Il
    Hong, Sung-Wook
    Kim, Kwang-sun
    Gho, Yong Song
    Jeon, Seong Gyu
    [J]. EXPERIMENTAL AND MOLECULAR MEDICINE, 2015, 47 : e183 - e183
  • [10] Efficient generation of complete sequences of MDR-encoding plasmids by rapid assembly of MinION barcoding sequencing data
    Li, Ruichao
    Xie, Miaomiao
    Dong, Ning
    Lin, Dachuan
    Yang, Xuemei
    Wong, Marcus Ho Yin
    Chan, Edward Wai-Chi
    Chen, Sheng
    [J]. GIGASCIENCE, 2018, 7 (03): : 1 - 9
12