Production of alkaline protease by Bacillus thuringiensis H 14 in aqueous two-phase systems

被引：0

作者：

Hotha, S ^{[1
]}

Banik, RM ^{[1
]}

机构：

[1] BANARAS HINDU UNIV,INST TECHNOL,SCH BIOCHEM ENGN,VARANASI 221005,UTTAR PRADESH,INDIA

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1997年 / 69卷 / 01期

关键词：

alkaline protease; enzyme purification and production; aqueous two-phase system (ATPS); product inhibition and partitioning;

D O I：

10.1002/(SICI)1097-4660(199705)69:1<5::AID-JCTB661>3.0.CO;2-I

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Alkaline protease production by Bacillus thuringiensis H 14 was studied in aqueous two-phase systems composed of PEG X (X = 9000, 6000, 4000) and potassium phosphate. The top phase is continuous and rich in PEG while the bottom phase is dispersed and is rich in phosphate, microbial cells being mainly retained in the bottom phase. The alkaline protease produced during fermentation, partitioned into the upper phase (about 80%) and total protease produced were about 2.80 and 2.26 times higher than that of homogeneous fermentation when the fermentations were carried out in aqueous two-phase systems from the beginning and made after 45 h of inoculation respectively.

引用

页码：5 / 10

页数：6

共 17 条

[1]

Albertsson P A, 1970, Adv Protein Chem, V24, P309, DOI 10.1016/S0065-3233(08)60244-2

[2]

Albertsson P. A., 1960, PARTITION CELL PARTI

[3] PARTICLE FRACTIONATION IN LIQUID 2-PHASE SYSTEMS - THE COMPOSITION OF SOME PHASE SYSTEMS AND THE BEHAVIOUR OF SOME MODEL PARTICLES IN THEM APPLICATION TO THE ISOLATION OF CELL WALLS FROM MICROORGANISMS [J].