Micropropagation of Banana: Reversion, Rooting, and Acclimatization of Hyperhydric Shoots

被引：9
作者：
El-Mahrouk, M. E. [1 ]
El-Shereif, A. R. [1 ]
Dewir, Y. H. [1 ,2 ]
Hafez, Y. M. [3 ]
Abdelaal, Kh A. [3 ]
El-Hendawy, S. [2 ,4 ]
Migdadi, H. [2 ]
Al-Obeed, R. S. [2 ]
机构：
[1] Kafrelsheikh Univ, Fac Agr, Dept Hort, Kafr Al Sheikh 33516, Egypt
[2] King Saud Univ, Coll Food & Agr Sci, Plant Prod Dept, Riyadh 11451, Saudi Arabia
[3] Kafrelsheikh Univ, Fac Agr, Dept Agr Bot, EPCRS Excellence Ctr,Plant Pathol & Biotechnol La, Kafr Al Sheikh 33516, Egypt
[4] Suez Canal Univ, Fac Agr, Agron Dept, Ismailia 41522, Egypt
来源：
HORTSCIENCE | 2019年 / 54卷 / 08期
关键词：
ANTIOXIDANT ENZYME-ACTIVITIES; WALL MECHANICAL-PROPERTIES; IN-VITRO CULTURE; LIPID-PEROXIDATION; TIP NECROSIS; REDUCING PROPERTIES; CARNATION INVITRO; OXIDATIVE STRESS; SALICYLIC-ACID; KEY FACTOR;
D O I：
10.21273/HORTSCI14036-19
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Hyperhydricity is a physiological disorder impacting plant growth and multiplication and acclimatization of regenerated plantlets. We report the use of calcium nitrate for reversion and acclimatization of banana ` Grand Naine' hyperhydric shoots cultured on Murashige and Skoog medium containing agar or gellan. Although 100% rooting of hyperhydric shoots occurred at all concentrations of calcium nitrate, only 50% rooting was recorded in the absence of calcium nitrate. Electrolyte leakage decreased significantly in the reverted banana tissues compared with the hyperhydric tissues. Histochemical staining for reactive oxygen species indicated that reverted banana tissues possess lower levels of both hydrogen peroxide (H2O2) and superoxide (O-2(-)) than do hyperhydric tissues. Rooting, growth, and survival of the reverted banana plantlets were significantly influenced by calcium nitrate concentrations as well as the type of gelling agent. Reverted banana plantlets in medium containing calcium nitrate (0.5-1 g.L-1) were acclimatized with 100% survival in a growing substrate of peatmoss and vermiculite (1: 1).
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页码：1384 / 1390
页数：7
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