Fungal nitrilases as biocatalysts: Recent developments

被引：42

作者：

Martinkova, Ludmila ^{[1
]}

Vejvoda, Vojtech ^{[1
]}

Kaplan, Ondrej ^{[1
]}

Kubac, David ^{[1
]}

Malandra, Anna ^{[1
,2
]}

Cantarella, Maria ^{[2
]}

Bezouska, Karel ^{[3
]}

Kren, Vladimir ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Microbiol, Ctr Biocatalysis & Biotransformat, CZ-14220 Prague, Czech Republic

[2] Univ Aquila, Dept Chem Chem Engn & Mat, I-67040 Laquila, Italy

[3] Charles Univ Prague, Fac Sci, Dept Biochem, CZ-12840 Prague, Czech Republic

来源：

BIOTECHNOLOGY ADVANCES | 2009年 / 27卷 / 06期

关键词：

Nitrilase; Filamentous fungi; Aspergillus; Fusarium; Nitrile biotransformation; Nitrile pollutant biodegradation; Immobilized biocatalysts; RHODOCOCCUS-RHODOCHROUS J1; ENZYME-PROMOTED DESYMMETRISATION; CYANIDE HYDRATASE GENE; FUSARIUM-SOLANI; HETEROLOGOUS EXPRESSION; SYNTHETIC APPLICATIONS; RICININE NITRILASE; GENOME SEQUENCE; MILD HYDROLYSIS; NICOTINIC-ACID;

D O I：

10.1016/j.biotechadv.2009.04.027

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Of the numerous putative fungal nitrilases available from protein databases only a few enzymes were purified and characterized The purified nitrilases from Fusarium solan., Fusarium oxysporum f sp. melonis and Aspergillus niger share a preference for (hetero)aromatic nitriles. temperature optima between 40 and 50 degrees C and pH optima in the slightly alkaline region On the other hand, they differ in their chemoselectivity, i.e. their tendency to produce amides as by-products. The production of fungal nitrilases is increased by up to three orders of magnitude on the addition of 2-cyanopyridine to the culture media The whole-cell and subcellular biocatalysts were immobilized by various methods (LentiKats (R): adsorption on hydrophobic or ion exchange resins. cross-linked enzyme aggregates) Operational stability was examined using continuous stirred membrane bioreactors Fungal nitrilases appear promising for biocatalytic applications and biodegradation of nitrile environmental contaminants (C) 2009 Elsevier Inc All rights reserved.

引用

页码：661 / 670

页数：10

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