Virus immobilization on biomaterial scaffolds through biotin-avidin interaction for improving bone regeneration

被引:10
作者
Hu, Wei-Wen [1 ,2 ,3 ]
Wang, Zhuo [1 ]
Krebsbach, Paul H. [1 ,3 ]
机构
[1] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, K1030,1011 N Univ Ave, Ann Arbor, MI 48109 USA
[2] Natl Cent Univ, Dept Chem & Mat Engn, 300 Jhongda Rd, Jhongli, Taoyuan County, Taiwan
[3] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
基金
美国国家卫生研究院;
关键词
tissue engineering; gene delivery; biotin-avidin; biomaterial scaffolds; bone regeneration; gene therapy; critical-sized bone defect; VIVO GENE-THERAPY; MORPHOGENETIC PROTEIN-7; DELIVERY; ADENOVIRUS; EXPRESSION; STRATEGIES; HYDROGELS; MATRICES; ANTIBODY; COLLAGEN;
D O I
10.1002/term.1774
中图分类号
Q813 [细胞工程];
学科分类号
摘要
To spatially control therapeutic gene delivery for potential tissue engineering applications, a biotin-avidin interaction strategy was applied to immobilize viral vectors on biomaterial scaffolds. Both adenoviral vectors and gelatin sponges were biotinylated and avidin was applied to link them in a virus-biotin-avidin-biotin-material (VBABM) arrangement. The tethered viral particles were stably maintained within scaffolds and SEM images illustrated that viral particles were evenly distributed in three-dimensional (3D) gelatin sponges. An in vivo study demonstrated that transgene expression was restricted to the implant sites only and transduction efficiency was improved using this conjugation method. For an orthotopic bone regeneration model, adenovirus encoding BMP-2 (AdBMP2) was immobilized to gelatin sponges before implanting into critical-sized bone defects in rat calvaria. Compared to gelatin sponges with AdBMP2 loaded in a freely suspended form, the VBABM method enhanced gene transfer and bone regeneration was significantly improved. These results suggest that biotin-avidin immobilization of viral vectors to biomaterial scaffolds may be an effective strategy to facilitate tissue regeneration. Copyright (c) 2013 John Wiley & Sons, Ltd.
引用
收藏
页码:E63 / E72
页数:10
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