Pre and post cloning characterization of a β-1,4-endoglucanase from Bacillus sp.

被引：17

作者：

Afzal, Sumra ^{[1
]}

Saleem, Mahjabeen ^{[1
]}

Yasmin, Riffat ^{[2
]}

Naz, Mamoona ^{[1
]}

Imran, Muhammad ^{[3
]}

机构：

[1] Univ Punjab, Inst Biochem & Biotechnol, Lahore 54590, Pakistan

[2] Sheikh Zayed Fed Postgrad Med Inst, Lahore 54590, Pakistan

[3] Univ Hlth Sci, Mol & Cell Biol Lab, Dept Physiol & Cell Biol, Lahore 54600, Pakistan

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 04期

关键词：

Bacillus sp; beta-1,4-Endoglucanase; Cloning; MOLECULAR-CLONING; ENDOGLUCANASE; GENE; CELLULASE;

D O I：

10.1007/s11033-009-9592-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Consistent with its precloning characterization from the cellulolytic Bacillus sp., beta-1,4-endoglucanase purified from the recombinant E. coli exhibited maximum activity at 60A degrees C and pH 7.0. It was highly specific for CMC hydrolysis, with stability up to 70A degrees C and over a pH range of 6.0-8.0. The K (m) and V (max) values for CMCase activity of the enzyme were 4.1 mg/ml and 25 mu mole/ml min(-1), respectively. The purified enzyme was a monomer of 65 kDa, as determined by SDS-PAGE. The presence of sucrose and IPTG in fermentation media increased the endoglucanase activity of the recombinant enzyme to 5.2-folds as compared with that of the actual one.

引用

页码：1717 / 1723

页数：7

共 29 条

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