An improved method for purification of recombinant truncated heme oxygenase-1 by expanded bed adsorption and gel filtration

被引：2
|
作者：
Hu, Hong-Bo [1 ]
Wang, Wei [1 ]
Han, Ling [1 ]
Zhou, Wen-Pu [1 ]
Zhang, Xue-Hong [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Key Lab Microbial Metab, Minist Educ, Shanghai 200240, Peoples R China
来源：
BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2007年 / 30卷 / 02期
关键词：
expanded bed adsorption; gel filtration; heme oxygenase-1; purification;
D O I：
10.1007/s00449-006-0103-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Recombinant truncated human heme oxygenase-1 (hHO-1) expressed in Escherichia coli was efficiently separated and purified from feedstock by DEAE-ion exchange expanded bed adsorption. Protocol optimization of hHO-1 on DEAE adsorbent resulted in adsorption in 0 M NaCl and elution in 150 mM NaCl at a pH of 8.5. The active enzyme fractions separated from the expanded bed column were further purified by a Superdex 75 gel filtration step. The specific hHO-1 activity increased from 0.82 +/- 0.05 to 24.8 +/- 1.8 U/mg during the whole purification steps. The recovery and purification factor of truncated hHO-1 of the whole purification were 72.7 +/- 4.7 and 30.2 +/- 2.3%, respectively. This purification process can decrease the demand on the preparation of feedstock and simplify the purification process.
引用
收藏
页码：87 / 90
页数：4
相关论文
共 50 条
  • [41] Enhanced high-level expression of soluble 1-aminocyclopropane-1-carboxylase synthase and rapid purification by expanded-bed adsorption
    Zhou, HQ
    Huxtable, S
    Xin, H
    Li, N
    PROTEIN EXPRESSION AND PURIFICATION, 1998, 14 (02) : 178 - 184
  • [42] Modulation of Glycolytic Shunting in Myeloid Cells Via Heme Oxygenase-1 Pathway Results in Enhanced T Cell Activation and Improved Survival in a Murine Glioma Model
    Kim, Lily H.
    Choi, John
    Nitta, Ryan
    Pant, Ayush
    Schonfeld, Ethan
    Li, Gordon
    Jackson, Christina
    Wang, Xinnan
    Lim, Michael
    NEUROSURGERY, 2025, 71 : 148 - 148
  • [43] Hydrophobic interaction expanded bed adsorption chromatography (HI-EBAC) based facile purification of recombinant Streptokinase from E-coli inclusion bodies
    Goyal, Deepika
    Sahoo, Debendra K.
    Sahni, Girish
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 850 (1-2): : 384 - 391
  • [44] SCINTILLATION PROXIMITY RT-PCR (SPRT-PCR) ASSAY FOR HEME OXYGENASE-1 (HO-1) AND HEME OXYGENASE-2 (HO-2) - A SIMPLE AND SENSITIVE METHOD TO ESTIMATE OXIDATIVE DAMAGE TO HUMAN-CELLS AND TISSUES
    KUTTY, RK
    BRIGHTWELL, J
    KUTTY, G
    NAGINENI, CN
    HOOKS, JJ
    CHADER, GJ
    WIGGERT, B
    FASEB JOURNAL, 1994, 8 (07): : A1400 - A1400
  • [45] Large-scale isolation and purification of R-phycoerythrin from red alga Palmaria palmata using the expanded bed adsorption method
    Wang, GC
    Sun, HB
    Fan, X
    Tseng, CK
    ACTA BOTANICA SINICA, 2002, 44 (05): : 541 - 546
  • [46] Induction of heme oxygenase-1 before conditioning results in improved survival and reduced graft-versus-host disease after experimental allogeneic bone marrow transplantation
    Gerbitz, A
    Ewing, P
    Wilke, A
    Schubert, T
    Eissner, G
    Dietl, B
    Andreesen, R
    Cooke, KR
    Holler, E
    BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 2004, 10 (07) : 461 - 472
  • [47] Large scale recovery and purification of periplasmic recombinant protein from E-coli using expanded bed adsorption chromatography followed by new ion exchange media
    Johansson, HJ
    Jagersten, C
    Shiloach, J
    JOURNAL OF BIOTECHNOLOGY, 1996, 48 (1-2) : 9 - 14
  • [48] Direct purification of recombinant hepatitis B core antigen from two different pre-conditioned unclarified Escherichia coli feedstocks via expanded bed adsorption chromatography
    Ng, Michelle Y. T.
    Tan, Wen Siang
    Abdullah, Norhafizah
    Ling, Tau Chuan
    Tey, Beng Ti
    JOURNAL OF CHROMATOGRAPHY A, 2007, 1172 (01) : 47 - 56
  • [49] Vascular Dysfunction in Experimental Diabetes is Associated With Cardiovascular Oxidative Stress, Loss in ecSOD Activity, Enos Uncoupling and Improved by Pentaerithrityl Tetranitrate - Role for heme oxygenase-1 and NRF2
    Oelze, Matthias
    Kroeller-Schoen, Swenja
    Kleinert, Hartmut
    Otto, Christian
    Heeren, Tjebo
    Steven, Sebastian
    Daub, Steffen
    Hausding, Michael
    Knorr, Maike
    Schulz, Eberhard
    Gori, Tommaso
    Wenzel, Philip
    Muenzel, Thomas
    Daiber, Andreas
    FREE RADICAL BIOLOGY AND MEDICINE, 2011, 51 : S64 - S64
  • [50] Expanded bed adsorption as a fast technique for the large-scale purification of the complete isoform pool of Ber e 1, the major allergen from Brazil nuts
    van Boxtel, EL
    van Koningsveld, GA
    Koppelman, SJ
    van den Broek, LAM
    Voragen, AGJ
    Gruppen, H
    MOLECULAR NUTRITION & FOOD RESEARCH, 2006, 50 (03) : 275 - 281
12345