Effect of Gamma Radiation and Oregano Essential Oil on Murein and ATP Concentration of Staphylococcus aureus

被引:19
作者
Caillet, Stephane [1 ]
Ursachi, Laura [1 ]
Shareck, Francois [1 ]
Lacroix, Monique [1 ]
机构
[1] INRS Inst Armand Frappier, Quebec City, PQ, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
cellular ATP; gamma irradiation; muropeptide; oregano oil; S; aureus; ESCHERICHIA-COLI O157-H7; PLANT ESSENTIAL OILS; PEPTIDOGLYCAN COMPOSITION; ANTIBACTERIAL ACTIVITY; ANTIMICROBIAL ACTIVITY; ENTEROCOCCUS-FAECALIS; CHEMICAL-COMPOSITION; FOOD; COMPONENTS; MECHANISM;
D O I
10.1111/j.1750-3841.2009.01368.x
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The study was carried out to evaluate the effects of gamma-irradiation alone or in combination with oregano essential oil on murein composition of Staphylococcus aureus and on the intracellular and extracellular concentration of ATP. The bacterial strain was treated with 3 irradiation doses: 1.2 kGy to induce cell damage, 2.9 kGy to obtain a viable but nonculturable state, and 3.5 kGy to cause cell death. Oregano essential oil was used at 0.010% and 0.013% (w/v), which is the minimum inhibitory concentration (MIC). All treatments had a significant effect (P <= 0.05) on the murein composition, although some muropeptides did not seem to be affected by the treatment. Each treatment influenced differently the relative percentage and number of muropeptides. There was a significant (P <= 0.05) correlation between the reduction of intracellular ATP and increase in extracellular ATP following treatment of the cells with oregano oil. The reduction of intracellular ATP was even more important when essential oil was combined with irradiation. Also, irradiation alone of S. aureus induced a significant decrease (P <= 0.05) of the internal ATP and a significant increase (P <= 0.05) of the external ATP. However, no significant difference (P > 0.05) was observed in ATP concentrations between different radiation doses. Transmission electron microscopic observation revealed that oregano oil and irradiation have an effect on cell wall structure.
引用
收藏
页码:M499 / M508
页数:10
相关论文
共 44 条
[1]   Chemical composition and inhibitory parameters of essential oil and extracts of Nandina domestica Thunb. to control food-borne pathogenic and spoilage bacteria [J].
Bajpai, Vivek K. ;
Rahman, Atiqur ;
Kang, Sun Chul .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2008, 125 (02) :117-122
[2]   ISOLATION AND CHARACTERIZATION OF THE PEPTIDOGLYCANS FROM SELECTED GRAM-POSITIVE AND GRAM-NEGATIVE PERIODONTAL PATHOGENS [J].
BARNARD, MR ;
HOLT, SC .
CANADIAN JOURNAL OF MICROBIOLOGY, 1985, 31 (02) :154-160
[3]   Combination of analytical and microbiological techniques to study the antimicrobial activity of a new active food packaging containing cinnamon or oregano against E-coli and S-aureus [J].
Becerril, R. ;
Gomez-Lus, R. ;
Goni, P. ;
Lopez, P. ;
Nerin, C. .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2007, 388 (5-6) :1003-1011
[4]  
BillotKlein D, 1996, BIOCHEM J, V313, P711, DOI 10.1042/bj3130711
[5]   ENZYMATIC INDUCTION OF DNA DOUBLE-STRAND BREAKS IN GAMMA-IRRADIATED ESCHERICHIA-COLI K-12 [J].
BONURA, T ;
SMITH, KC ;
KAPLAN, HS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (11) :4265-4269
[6]   A spectrum of changes occurs in peptidoglycan composition of glycopeptide-intermediate clinical Staphylococcus aureus isolates [J].
Boyle-Vavra, S ;
Labischinski, H ;
Ebert, CC ;
Ehlert, K ;
Daum, RS .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (01) :280-287
[7]   Antibacterial activity of selected plant essential oils against Escherichia coli O157:H7 [J].
Burt, SA ;
Reinders, RD .
LETTERS IN APPLIED MICROBIOLOGY, 2003, 36 (03) :162-167
[8]  
Cabiscol Elisa, 2000, International Microbiology, V3, P3
[9]   Influence of antimicrobial compounds and modified atmosphere packaging on radiation sensitivity of Listeria monocytogenes present in ready-to-use carrots (Daucus carota) [J].
Caillet, S ;
Millette, M ;
Turgis, M ;
Salmieri, S ;
Lacroix, M .
JOURNAL OF FOOD PROTECTION, 2006, 69 (01) :221-227
[10]   Effect of gamma radiation and oregano essential oil on murein and ATP concentration of Escherichia coli O157:H7 [J].
Caillet, S ;
Shareck, F ;
Lacroix, M .
JOURNAL OF FOOD PROTECTION, 2005, 68 (12) :2571-2579