Machine-Learning Single-Stranded DNA Nanoparticles for Bacterial Analysis

被引：19

作者：

Nandu, Nidhi ^{[1
]}

Smith, Christopher W. ^{[1
]}

Uyar, Taha Bilal ^{[1
]}

Chen, Yu-Sheng ^{[1
]}

Kachwala, Mahera J. ^{[1
]}

He, Muhan ^{[1
]}

Yigit, Mehmet, V ^{[1
,2
]}

机构：

[1] SUNY Albany, Dept Chem, Albany, NY 12222 USA

[2] SUNY Albany, RNA Inst, Albany, NY 12222 USA

来源：

ACS APPLIED NANO MATERIALS | 2020年 / 3卷 / 12期

基金：

美国食品与农业研究所; 美国国家卫生研究院;

关键词：

sensor array; DNA; bacterial detection; nanographene oxide (nGO); MoS2; fluorescence; GRAPHENE OXIDE; ADSORPTION; ARRAY;

D O I：

10.1021/acsanm.0c03001

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A two-dimensional nanoparticle-single-stranded DNA (ssDNA) array has been assembled for the detection of bacterial species using machine-learning (ML) algorithms. Out of 60 unknowns prepared from bacterial lysates, 54 unknowns were predicted correctly. Furthermore, the nanosensor array, supported by ML algorithms, was able to distinguish wild-type Escherichia coli from its mutant by a single gene difference. In addition, the nanosensor array was able to distinguish untreated wild-type E. coli from those treated with antimicrobial drugs. This work demonstrates the potential of nanoparticle-ssDNA arrays and ML algorithms for the discrimination and identification of complex biological matrixes.

引用

收藏

页码：11709 / 11714

页数：6

相关论文

共 31 条

[11] Homologous miRNA Analyses Using a Combinatorial Nanosensor Array with Two-Dimensional Nanoparticles [J].

Hizir, Mustafa Salih ;

Nandu, Nidhi ;

Yigit, Mehmet V. .

ANALYTICAL CHEMISTRY, 2018, 90 (10) :6300-6306

[12] Universal sensor array for highly selective system identification using two-dimensional nanoparticles [J].

Hizir, Mustafa Salih ;

Robertson, Neil M. ;

Balcioglu, Mustafa ;

Alp, Esma ;

Rana, Muhit ;

Yigit, Mehmet V. .

CHEMICAL SCIENCE, 2017, 8 (08) :5735-5745

[13] Simultaneous Detection of Circulating OncomiRs from Body Fluids for Prostate Cancer Staging Using Nanographene Oxide [J].

Hizir, Mustafa Salih ;

Balcioglu, Mustafa ;

Rana, Muhit ;

Robertson, Neil M. ;

Yigit, Mehmet V. .

ACS APPLIED MATERIALS & INTERFACES, 2014, 6 (17) :14772-14778

[14] Janus DNA orthogonal adsorption of graphene oxide and metal oxide nanoparticles enabling stable sensing in serum [J].

Liu, Biwu ;

Ma, Lingzi ;

Huang, Zhicheng ;

Hu, Hao ;

Wu, Peng ;

Liu, Juewen .

MATERIALS HORIZONS, 2018, 5 (01) :65-69

[15] DNA adsorbed on graphene and graphene oxide: Fundamental interactions, desorption and applications [J].

Liu, Biwu ;

Salgado, Shehan ;

Maheshwari, Vivek ;

Liu, Juewen .

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2016, 26 :41-49

[16] The effects of kanamycin concentration on gene transcription levels in Escherichia coli [J].

Liu, Xiaozhen ;

Li, Na ;

Jia, Minjun ;

Zhang, Shuangli ;

Niu, Hao ;

Li, Qiang ;

Gu, Pengfei .

3 BIOTECH, 2020, 10 (03)

[17] Comparison of MoS2, WS2, and Graphene Oxide for DNA Adsorption and Sensing [J].

Lu, Chang ;

Liu, Yibo ;

Ying, Yibin ;

Liu, Juewen .

LANGMUIR, 2017, 33 (02) :630-637

[18] Enhanced Quality Control in Pharmaceutical Applications by Combining Raman Spectroscopy and Machine Learning Techniques [J].

Martinez, J. C. ;

Guzman-Sepulveda, J. R. ;

Bolanoz Evia, G. R. ;

Cordova, T. ;

Guzman-Cabrera, R. .

INTERNATIONAL JOURNAL OF THERMOPHYSICS, 2018, 39 (06)

[19] Lipoprotein Lpp regulates the mechanical properties of the E. coli cell envelope [J].

Mathelie-Guinlet, Marion ;

Asmar, Abir T. ;

Collet, Jean-Francois ;

Dufrene, Yves F. .

NATURE COMMUNICATIONS, 2020, 11 (01)

[20] Molecular afterglow imaging with bright, biodegradable polymer nanoparticles [J].

Miao, Qingqing ;

Xie, Chen ;

Zhen, Xu ;

Lyu, Yan ;

Duan, Hongwei ;

Liu, Xiaogang ;

Jokerst, Jesse V. ;

Pu, Kanyi .

NATURE BIOTECHNOLOGY, 2017, 35 (11) :1102-+

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