The Expression of Markers Related to Ovarian Germline Stem Cells in the Mouse Ovarian Surface Epithelium and the Correlation with Notch Signaling Pathway

被引：21

作者：

Pan, Zezheng ^{[1
,2
]}

Sun, Mengli ^{[1
,3
]}

Li, Jia ^{[1
]}

Zhou, Fangyue ^{[1
]}

Liang, Xia ^{[1
]}

Huang, Jian ^{[1
]}

Zheng, Tuochen ^{[4
]}

Zheng, Liping ^{[1
]}

Zheng, Yuehui ^{[1
]}

机构：

[1] Nanchang Univ, Med Teaching Lab Ctr, Jiangxi Med Coll, Nanchang 330031, Jiangxi, Peoples R China

[2] Nanchang Univ, Jiangxi Med Coll, Fac Basic Med Sci, Nanchang 330031, Jiangxi, Peoples R China

[3] Chinese Peoples Liberat Army Gen Hosp, Coll Life Sci, Beijing 100853, Peoples R China

[4] Nanchang Univ, Clin Med Coll 1, Nanchang 330031, Jiangxi, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2015年 / 37卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Ovarian germline stem cells; Notch signaling; DAPT; Mice; DROSOPHILA OVARY; NICHE FORMATION; DIFFERENTIATION; GROWTH; PROLIFERATION; LINE; PROGNOSIS; BIOPSIES; PORCINE; BRAIN;

D O I：

10.1159/000438586

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Ovarian germline stem cells (OGSCs) have been shown to mainly exist in the ovarian surface epithelium (OSE), but the activity changes of germline stem cells during different reproductive stages and the potential regulatory signaling pathway are still unknown. The Notch signaling pathway plays a key role in cell development, primordial follicles and stem cell proliferation. However, whether it plays a role in the proliferation of OGSCs is unknown. Here, we analyzed the activity changes of germline stem cells and the correlation between germline stem cells and the Notch signaling pathway. Methods: The expression of germline stem cell markers Mvh, Ooc4 and the Notch molecules Notch1, Hes1, and Hes5 were detected during 3 days (3d), and 2, 12, 20 months (2m, 12m, 20m) mouse ovarian surface epithelium samples. DAPT, a specific inhibitor of the Notch pathway, was used to observe the influence of Notch signaling in the germline stem cells. Results: The results showed that the levels of MVH and OCT4 decreased substantially with reproductive age in ovarian surface epithelium, and the same tendency was detected in the Notch signaling molecules Notch1, Hes1 and Hes5. Dual-IF results showed that the germline stem cell markers were co-expressed with Notch molecules in the ovarian surface epithelium. While, the expression of MVH and OCT4 were reduced when the ovaries were treated with DAPT and the levels were attenuated with increasing dose of DAPT. Conclusion: Taken together, our results indicate that the viability of OGSCs decreased with the age of the mouse ovaries, and the activity of OGSCs in the ovarian surface epithelium may be related to the Notch signaling pathway. (C) 2015 The Author(s) Published by S. Karger AG, Basel

引用

页码：2311 / 2322

页数：12

共 46 条

[1] Location and characterization of female germline stem cells (FGSCs) in juvenile porcine ovary [J].

Bai, Y. ;

Yu, M. ;

Hu, Y. ;

Qiu, P. ;

Liu, W. ;

Zheng, W. ;

Peng, S. ;

Hua, J. .

CELL PROLIFERATION, 2013, 46 (05) :516-528

[2] Identification of self-replicating multipotent progenitors in the embryonic nervous system by high Notch activity and Hes5 expression [J].

Basak, Onur ;

Taylor, Verdon .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2007, 25 (04) :1006-1022

[3] Germline cells in ovarian surface epithelium of mammalians: a promising notion [J].

Celik, Onder ;

Celik, Ebru ;

Turkcuoglu, Ilgin ;

Yilmaz, Ercan ;

Simsek, Yavuz ;

Tiras, Bulent .

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2012, 10

[4] The Jagged-1/Notch-1/Hes-1 Pathway Is Involved in Intestinal Adaptation in a Massive Small Bowel Resection Rat Model [J].

Chen, Guoqing ;

Sun, Lihua ;

Yu, Min ;

Meng, Dan ;

Wang, Wensheng ;

Yang, Yang ;

Yang, Hua .

DIGESTIVE DISEASES AND SCIENCES, 2013, 58 (09) :2478-2486

[5] Detection of Four Germ Cell Markers in Rats during Testis Morphogenesis: Differences and Similarities with Mice [J].

Encinas, Giselly ;

Zogbi, Camila ;

Stumpp, Taiza .

CELLS TISSUES ORGANS, 2012, 195 (05) :443-455

[6] Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine [J].

Fan, Y. X. ;

Gu, C. H. ;

Zhang, Y. L. ;

Zhong, B. S. ;

Wang, L. Z. ;

Zhou, Z. R. ;

Wang, Z. Y. ;

Jia, R. X. ;

Wang, F. .

GENETICS AND MOLECULAR RESEARCH, 2013, 12 (04) :6067-6079

[7] Expression pattern of Notch intracellular domain (NICD) and Hes-1 in preneoplastic and neoplastic human oral squamous epithelium: their correlation with c-Myc, clinicopathological factors and prognosis in Oral cancer [J].

Gokulan, Ravindran ;

Halagowder, Devaraj .

MEDICAL ONCOLOGY, 2014, 31 (08)

[8] Notch Signaling Plays a Critical Role in Motility and Differentiation of Human First-Trimester Cytotrophoblasts [J].

Haider, Sandra ;

Meinhardt, Gudrun ;

Velicky, Philipp ;

Otti, Gerlinde R. ;

Whitley, Guy ;

Fiala, Christian ;

Pollheimer, Juergen ;

Knoefler, Martin .

ENDOCRINOLOGY, 2014, 155 (01) :263-274

[9] GSK3 inhibitor-BIO regulates proliferation of female germline stem cells from the postnatal mouse ovary [J].

Hu, Y. ;

Bai, Y. ;

Chu, Z. ;

Wang, J. ;

Wang, L. ;

Yu, M. ;

Lian, Z. ;

Hua, J. .

CELL PROLIFERATION, 2012, 45 (04) :287-298

[10] HES and HERP families: Multiple effectors of the Notch signaling pathway [J].

Iso, T ;

Kedes, L ;

Hamamori, Y .

JOURNAL OF CELLULAR PHYSIOLOGY, 2003, 194 (03) :237-255

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