Isolation and characterization of highly polymorphic microsatellites in tea (Camellia sinensis)

被引:40
作者
Freeman, S
West, J
James, C
Lea, V
Mayes, S
机构
[1] NIAB, Cambridge CB3 0LE, England
[2] Univ Cambridge, Dept Genet, Cambridge CB2 3EH, England
[3] HRI, W Malling ME19 6BF, Kent, England
[4] Univ Nottingham, Div Agr & Environm, Loughborough LE12 5RD, Leics, England
来源
MOLECULAR ECOLOGY NOTES | 2004年 / 4卷 / 03期
关键词
Camellia; diversity; identity; microsatellite; tea;
D O I
10.1111/j.1471-8286.2004.00682.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Relatively little is known about the diversity and origins of tea. The highest value tea products are sold on the basis of their region of origin but there are currently no methods available to verify the claims made on packages. We have developed 15 microsatellite loci for tea. These have been evaluated for polymorphism in a set of tea clones to determine their usefulness for authentication purposes. The majority of the microsatellites developed proved to be highly polymorphic both between and within different geographical origins and offer the potential to investigate the population genetics and genetic origins of tea.
引用
收藏
页码:324 / 326
页数:3
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