Chronic administration of methionine and/or methionine sulfoxide alters oxidative stress parameters and ALA-D activity in liver and kidney of young rats

被引：23

作者：

Pereira Soares, Mayara Sandrielly . ^{[1
]}

Oliveira, Pathise Souto ^{[2
]}

Debom, Gabriela Nogueira ^{[1
]}

Mattos, Bruna da Silveira ^{[1
]}

Polachini, Carla Roberta ^{[3
]}

Baldissarelli, Jucimara ^{[3
]}

Morsch, Vera Maria ^{[3
]}

Chitolina Schetinger, Maria Rosa ^{[3
]}

Tavares, Rejane Giacomelli ^{[1
]}

Stefanello, Francieli Moro ^{[2
]}

Spanevello, Roselia Maria ^{[1
]}

机构：

[1] Univ Fed Pelotas, Ctr Ciencias Quim Farmaceut & Alimentos, Lab Neuroquim Inflamacao & Canc, Programa Posgrad Bioquim & Bioprospeccao, Campus Univ S-N, BR-96010900 Pelotas, RS, Brazil

[2] Univ Fed Pelotas, Ctr Ciencias Quim Farmaceut & Alimentos, Lab Biomarcadores, Programa Posgrad Bioquim & Bioprospeccao, Campus Univ S-N, Pelotas, RS, Brazil

[3] Univ Fed Santa Maria, Ctr Ciencias Nat & Exatas, Lab Enzimol Toxicol, Programa Posgrad Bioquim Toxicol, Campus Univ S-N, Santa Maria, RS, Brazil

来源：

AMINO ACIDS | 2017年 / 49卷 / 01期

关键词：

Methionine; Methionine sulfoxide; Oxidative stress; Delta aminolevulinic dehydratase; DELTA-AMINOLEVULINATE DEHYDRATASE; ADENOSYLTRANSFERASE I/III DEFICIENCY; LIPID-PEROXIDATION; HYPERMETHIONINEMIA; ANTIOXIDANTS; METABOLISM; PROTEINS; RESIDUES; DAMAGE; FAT;

D O I：

10.1007/s00726-016-2340-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High levels of methionine (Met) and methionine sulfoxide (MetO) are found in several genetic abnormalities. Oxidative stress is involved in the pathophysiology of many inborn errors of metabolism. However, little is known about the role of oxidative damage in hepatic and renal changes in hypermethioninemia. We investigated the effect of chronic treatment with Met and/or MetO on oxidative stress parameters in liver and kidney, as lipid peroxidation (TBARS), total sulfhydryl content (SH), reactive oxygen species (ROS) and enzymes activities superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and delta aminolevulinic dehydratase (ALA-D). Serum biochemical parameters were evaluated. Wistar rats were treated daily with two subcutaneous injections of saline (control), Met (0.2-0.4 g/kg), MetO (0.05-0.1 g/kg) and the association between these (Met plus MetO) from the 6th to the 28th day of life. Our data demonstrated an increase of glucose and urea levels in all experimental groups. Cholesterol (MetO and Met plus MetO) were decreased and triglycerides (MetO) were increased. SOD (MetO and Met plus MetO) and CAT (Met, MetO and Met plus MetO) activities were decreased, while GPx was enhanced by MetO and Met plus MetO treatment in liver. In kidney, we observed a reduction of SH levels, SOD and CAT activities and an increase of TBARS levels in all experimental groups. ROS levels in kidney were increased in MetO and Met plus MetO groups. ALA-D activity was enhanced in liver (MetO and Met plus MetO) and kidney (Met plus MetO). These findings help to understand the pathophysiology of hepatic and renal alterations present in hypermethioninemia.

引用

页码：129 / 138

页数：10

共 44 条

[1]

AEBI H, 1984, METHOD ENZYMOL, V105, P121

[2] Changes in thiol content and expression of glutathione redox system genes in the hippocampus and cerebellum in Alzheimer's disease [J].