Vindoline synthesis in in vitro shoot cultures of Catharanthus roseus

被引:24
|
作者
Hernández-Domínguez, E [1 ]
Campos-Tamayo, F [1 ]
Vázquez-Flota, F [1 ]
机构
[1] Ctr Invest Cient Yucatan, Unidad Bioquim & Biol Mol Plantas, Chuburna 97200, Merida Yucatan, Mexico
关键词
alkaloids; Catharanthus roseus; shoot cultures; vindoline;
D O I
10.1023/B:BILE.0000023028.21985.07
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Vindoline, the major alkaloid in cultures of Catharanthus roseus shoots, reached 2 mg g(-1) dry wt after 27 d in culture. Maximal vindoline accumulation coincided with maximum activities of deacetoxyvindoline 4-hydroxylase, deacetylvindoline acetyl-CoA acetyl transferase and tryptophan decarboxylase. Shoot exposure to jasmonate shortened the time required for the maximal vindoline accumulation to 14 d.
引用
收藏
页码:671 / 674
页数:4
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