Adzuki bean: A new resource of biocatalyst for asymmetric reduction of aromatic ketones with high stereoselectivity and substrate tolerance

被引：36

作者：

Xie, Yan ^{[1
]}

Xu, Jian-He ^{[1
]}

Lu, Wen-Ya ^{[2
]}

Lin, Guo-Qiang ^{[2
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Bioproc, Shanghai 200237, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Adzuki bean; Phaseolus angularis; Biocatalyst; Asymmetric reduction; Substrate tolerance; GEOTRICHUM-CANDIDUM; DAUCUS-CAROTA; CARBONYL REDUCTASE; ACETONE POWDER; HYDROGENATION;

D O I：

10.1016/j.biortech.2008.11.054

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A new resource of biocatalyst for asymmetric reduction of aromatic ketones has been discovered for the first time from a common plant seed, adzuki bean, i.e. Phaseolus angularis (Willd.) W.F. Wight. The study investigated the best methods to prepare the biocatalyst and its ability to reduce ketones. Our results indicated that the biocatalyst from adzuki bean could reduce various aromatic ketones at relatively high concentrations (e.g. 100 mM). exhibiting excellent stereoselectivity (>98% e.e.). In addition, it was found that NADPH acts as the reducing cofactor, which can be regenerated by the crude enzyme system itself using glucose as an auxiliary substrate. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2463 / 2468

页数：6