Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway

被引：47

作者：

Hirokawa, Yasutaka ^{[1
]}

Maki, Yuki ^{[1
]}

Tatsuke, Tsuneyuki ^{[1
]}

Hanai, Taizo ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Syst Biosci, Lab Bioinformat, Higashi Ku, 804 Westwing,3-1-1 Maidashi, Fukuoka 8128582, Japan

来源：

METABOLIC ENGINEERING | 2016年 / 34卷

基金：

日本科学技术振兴机构;

关键词：

Cyanobacteria; Synthetic metabolic pathway; 1,3-propanediol production; Dihydroxyacetone phosphate; Pseudovitamin B-12; PHOTOSYNTHETIC PRODUCTION; ENGINEERING CYANOBACTERIA; ISOPROPANOL PRODUCTION; KLEBSIELLA-PNEUMONIAE; METHIONINE SYNTHASE; GLYCOGEN PRODUCTION; BIOFUEL PRODUCTION; ACID PRODUCTION; OPTIMIZATION; EXPRESSION;

D O I：

10.1016/j.ymben.2015.12.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Production of chemicals directly from carbon dioxide using light energy is an attractive option for a sustainable future. The 1,3-propanediol (1,3-PDO) production directly from carbon dioxide was achieved by engineered Synechococcus elongatus PCC 7942 with a synthetic metabolic pathway. Glycerol dehydratase catalyzing the conversion of glycerol to 3-hydroxypropionaldehyde in a coenzyme B-12-dependent manner worked in S. elongatus PCC 7942 without addition of vitamin B-12, suggesting that the intrinsic pseudovitamin B12 served as a substitute of coenzyme B12. The highest titers of 1,3-PDO (3.79 +/- 0.23 mM; 288 +/- 17.7 mg/L) and glycerol (12.62 +/- 1.55 mM; 1.16 +/- 0.14 g/L), precursor of 1,3-PDO, were reached after 14 days of culture under optimized conditions in this study. (C) 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

引用

页码：97 / 103

页数：7

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