XL-MS: Protein cross-linking coupled with mass spectrometry

被引：81

作者：

Holding, Andrew N. ^{[1
]}

机构：

[1] Univ Cambridge, Canc Res UK Cambridge Inst, Li Ka Shing Ctr, Cambridge CB2 0RE, England

来源：

METHODS | 2015年 / 89卷

基金：

英国医学研究理事会;

关键词：

Proteomics; Mass spectrometry; Structure; Quantitation; Systems biology; Cross-linking; XL-MS; LINKED PEPTIDES; RAPID IDENTIFICATION; SELECTIVE ENRICHMENT; STRUCTURAL-ANALYSIS; ACIDIC RESIDUES; PROTEOMICS; REAGENTS; PROBES; RNA; PURIFICATION;

D O I：

10.1016/j.ymeth.2015.06.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

With the continuing trend to study larger and more complex systems, the application of protein cross-linking coupled with mass spectrometry (XL-MS) provides a varied toolkit perfectly suited to achieve these goals. By freezing the transient interactions through the formation of covalent bonds, XL-MS provides a vital insight into both the structure and organization of proteins in a wide variety of conditions. This review covers some of the established methods that underpin the field alongside the more recent developments that hold promise to further realize its potential in new directions. (C) 2015 The Author. Published by Elsevier Inc. This is an open access article under the CC BY license.

引用

页码：54 / 63

页数：10

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