SP1-induced AFAP1-AS1 contributes to proliferation and invasion by regulating miR-497-5p/CELF1 pathway in nasopharyngeal carcinoma

被引：9

作者：

Jin, Hui ^{[1
]}

Liang, Gengtian ^{[1
]}

Yang, Liping ^{[1
]}

Liu, Li ^{[1
]}

Wang, Binru ^{[1
]}

Yan, Fengqin ^{[2
,3
]}

机构：

[1] Wuhan Third Hosp, Dept Otolaryngol, Wuhan 430000, Hubei, Peoples R China

[2] Univ Chinese Acad Sci, Dept Head & Neck Radiat Therapy, Zhejiang Canc Hosp, Canc Hosp, 1 Banshan Rd, Hangzhou 310021, Zhejiang, Peoples R China

[3] Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310021, Zhejiang, Peoples R China

来源：

HUMAN CELL | 2021年 / 34卷 / 02期

关键词：

Nasopharyngeal carcinoma; AFAP1-AS1; SP1; miR-497-5p; LONG NONCODING RNAS; CELL-PROLIFERATION; TUMOR-GROWTH; METASTASIS; CANCER; PROGRESSION; EXPRESSION;

D O I：

10.1007/s13577-020-00475-y

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nasopharyngeal carcinoma is a type of otolaryngological malignancy with high incidence. Long non-coding RNAs (lncRNAs) are closely related to nasopharyngeal carcinoma. LncRNA AFAP1-AS1 (AFAP1-AS1) has been found to play important roles in nasopharyngeal carcinoma progression and poor prognosis. However, the mechanism underlying AFAP1-AS1 in regulating nasopharyngeal carcinoma is still unclear. In current study, AFAP1-AS1 was found to be up-regulated in nasopharyngeal carcinoma tissues and cells. AFAP1-AS1 overexpression and knockdown were conducted in nasopharyngeal carcinoma cells. The results proved that AFAP1-AS1 promoted the survival and migration of nasopharyngeal carcinoma cells. Additionally, specificity protein 1 (SP1) was enhanced in nasopharyngeal carcinoma tissues and cells, and induced AFAP1-AS1 expression. The interaction between AFAP1-AS1 and miR-497-5p was confirmed. AFAP1-AS1 was demonstrated to regulate CELF1, a target gene of miR-497-5p. Further functional analysis revealed that AFAP1-AS1 knockdown attenuated SP1-induced nasopharyngeal carcinoma progression. These results indicate that SP1-induced AFAP1-AS1 facilitates nasopharyngeal carcinoma progression by regulating miR-497-5p/CELF1 pathway, which provides a new target for nasopharyngeal carcinoma treatment.

引用

页码：491 / 501

页数：11

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