Physicochemical characterization and antitumor activity in vitro of a selenium polysaccharide from Pleurotus ostreatus

Selenium-enriched polysaccharides have been gaining great attention for their antitumor activity in recent years. In this study, a novel seleniumpolysaccharide fraction (Se-POP-3) produced by Pleurotus ostreatuswas characterized and its antitumor activity explored at cellular level. Results showed that Se-POP-3 has 25.9 mu g/g of selenium, an average molecular weight of 16,106 Da, and is mainly composed of mannose, glucose and galactose with a molar ratio of 1.7:49.6:2.4. Spectra analysis revealed Se-POP- 3 as a pyranopolysaccharide linked by alpha-glycoside bonds in the main chain, and selenium may occur in the form of C-O-Se and Se=O. A single sphere of Se-POP-3 has 50-60 nm in aqueous solution, even though it can agglomerate to form larger spherical structures. In vitro experiments with cancer and normal cell lines showed that Se-POP-3 can induce apoptosis and inhibit migration of cancer cells. Potential anticancer mechanism is that Se-POP-3 can disrupt the Bax/Bcl-2 protein ratio and inhibit the epithelial-to-mesenchymal transition (EMT) in cancer cells. Se-POP-3 showed no significant effect on the growth of normal cell lines. Se-POP-3 showed great potential as a broad-spectrum antitumor agent and dietary supplement. (c) 2020 Published by Elsevier B.V.