A fibrinolytic enzyme from a marine green alga, Codium latum

被引：41

作者：

Matsubara, K

Hori, K

Matsuura, Y

Miyazawa, K

机构：

[1] Okayama Prefectural Univ, Fac Hlth & Welf Sci, Dept Nutr Sci, Okayama 7191197, Japan

[2] Hiroshima Univ, Fac Appl Biol Sci, Higashihiroshima 7398528, Japan

来源：

PHYTOCHEMISTRY | 1999年 / 52卷 / 06期

关键词：

Codium latum; fibrinogenolytic activity; fibrinolytic activity; marine alga; molecular weight; optimum pH; protease activity; purification; serine protease;

D O I：

10.1016/S0031-9422(99)00356-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fibrinolytic enzyme was isolated from a marine green alga, Codium latum, and designated C. latum protease (CLP). It also had fibrinogenolytic activity, hydrolyzing A alpha, B beta and gamma chains with preference in this order. As CLP hydrolyzed oxidized insulin B chain at position Arg(22)-Gly(23), and the peptide map of lysozyme digested with CLP was similar to that with trypsin, CLP would be expected to have a high substrate specificity, similar to that of trypsin. Protease activity peaked at pH 10, and was completely inhibited by diisopropyl fluorophosphate (DFP). Therefore, we conclude that CLP is a trypsin-like serine protease. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：993 / 999

页数：7

共 17 条

[1] THE FIBRIN PLATE METHOD FOR ESTIMATING FIBRINOLYTIC ACTIVITY [J].