Genome sequence of the cultivated cotton Gossypium arboreum

被引：724

作者：

Li, Fuguang ^{[1
]}

Fan, Guangyi ^{[2
]}

Wang, Kunbo ^{[1
]}

Sun, Fengming ^{[2
]}

Yuan, Youlu ^{[1
]}

Song, Guoli ^{[1
]}

Li, Qin ^{[3
]}

Ma, Zhiying ^{[4
]}

Lu, Cairui ^{[1
]}

Zou, Changsong ^{[1
]}

Chen, Wenbin ^{[2
]}

Liang, Xinming ^{[2
]}

Shang, Haihong ^{[1
]}

Liu, Weiqing ^{[2
]}

Shi, Chengcheng ^{[2
]}

Xiao, Guanghui ^{[3
]}

Gou, Caiyun ^{[2
]}

Ye, Wuwei ^{[1
]}

Xu, Xun ^{[2
]}

Zhang, Xueyan ^{[1
]}

Wei, Hengling ^{[1
]}

Li, Zhifang ^{[1
]}

Zhang, Guiyin ^{[4
]}

Wang, Junyi ^{[2
]}

Liu, Kun ^{[1
]}

Kohel, Russell J. ^{[5
]}

Percy, Richard G. ^{[5
]}

Yu, John Z. ^{[5
]}

Zhu, Yu-Xian ^{[3
]}

Wang, Jun ^{[2
,6
,7
,8
,9
,10
]}

Yu, Shuxun ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China

[2] BGI Shenzhen, Shenzhen, Peoples R China

[3] Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

[4] Agr Univ Hebei, Key Lab Crop Germplasm Resources Hebei, Baoding, Peoples R China

[5] USDA ARS, Crop Germplasm Res Unit, Southern Plains Agr Res Ctr, College Stn, TX USA

[6] Univ Copenhagen, Dept Biol, Copenhagen, Denmark

[7] King Abdulaziz Univ, Jeddah 21413, Saudi Arabia

[8] Macau Univ Sci & Technol, Macau, Peoples R China

[9] Univ Hong Kong, Dept Med, Hong Kong, Hong Kong, Peoples R China

[10] Univ Hong Kong, State Key Lab Pharmaceut Biotechnol, Hong Kong, Hong Kong, Peoples R China

来源：

NATURE GENETICS | 2014年 / 46卷 / 06期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

CELL ELONGATION; DRAFT GENOME; EVOLUTION; ARABIDOPSIS; FIBER; BIOSYNTHESIS; DYNAMICS; ETHYLENE; SORGHUM; GENUS;

D O I：

10.1038/ng.2987

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The complex allotetraploid nature of the cotton genome (AADD; 2n = 52) makes genetic, genomic and functional analyses extremely challenging. Here we sequenced and assembled the Gossypium arboreum (AA; 2n = 26) genome, a putative contributor of the A subgenome. A total of 193.6 Gb of clean sequence covering the genome by 112.6-fold was obtained by paired-end sequencing. We further anchored and oriented 90.4% of the assembly on 13 pseudochromosomes and found that 68.5% of the genome is occupied by repetitive DNA sequences. We predicted 41,330 protein-coding genes in G. arboreum. Two whole-genome duplications were shared by G. arboreum and Gossypium raimondii before speciation. Insertions of long terminal repeats in the past 5 million years are responsible for the twofold difference in the sizes of these genomes. Comparative transcriptome studies showed the key role of the nucleotide binding site (NBS)-encoding gene family in resistance to Verticillium dahliae and the involvement of ethylene in the development of cotton fiber cells.

引用

页码：567 / 572

页数：6