High-Capacity Strong Cation Exchange Resin Based on Reutilization of An Inactivated Immobilized Enzyme and Its Adsorption Properties for Lysozyme

被引：1

作者：

Yuan Jing-Xiang ^{[1
]}

Wang Chao-Zhan ^{[1
]}

Wei Yin-Mao ^{[1
]}

机构：

[1] Northwest Univ Xian, Coll Chem & Mat Sci, Synthet & Nat Funct Mol Chem Minist Educ, Key Lab, Xian 710127, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2016年 / 44卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Ion-exchange resin; Solid wastes; Surface-initiated atom transfer radical polymerization; Adsorption; Lysozyme; TRANSFER RADICAL POLYMERIZATION; PENICILLIN-G ACYLASE; 2,4-DICHLOROPHENOXYACETIC ACID; PROTEIN ADSORPTION; ION-EXCHANGERS; CHITOSAN BEADS; REACTIVATION; ADSORBENT; MEMBRANE; CHROMATOGRAPHY;

D O I：

10.11895/j.issn.0253-3820.160296

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new cation exchange resin was prepared based on surface. initiated atom transfer radical polymerization (SI. ATRP) of poly (sodium 4-styrene sulfonate) (PNASS) at 30 degrees C for 3 h from inactivated immobilized enzymes. The cation exchange resin was characterized by Fourier transform infrared spectrometry (FT-IR). Lysozyme was selected as a model protein to evaluate the adsorption performance of the as. prepared cation exchange resin. The effects of protein concentration, ionic strength, organic solvent, contacting time and temperature on protein adsorption were investigated. The results showed that the cation exchange resin exhibited very good performance for the adsorption of lysozyme with a high capacity (240 mg/g) at room temperature and fast speed (within 30 min), which were much better than those reported in previously reported works. The adsorption isotherms could be better fitted by Langmuir model than Freundlich model and the adsorption process followed the pseudo. second. order kinetic model.

引用

页码：1892 / 1899

页数：8

共 38 条

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