Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells

被引：54

作者：

Takimoto, Jeffrey K. ^{[1
]}

Adams, Katrina L. ^{[1
]}

Xiang, Zheng ^{[1
]}

Wang, Lei ^{[1
]}

机构：

[1] Salk Inst Biol Studies, Jack H Skirball Ctr Chem Biol & Prote, La Jolla, CA 92037 USA

来源：

MOLECULAR BIOSYSTEMS | 2009年 / 5卷 / 09期

关键词：

COGNATE TRANSFER-RNA; GENETIC-CODE; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; STRUCTURAL BASIS; PROTEINS;

D O I：

10.1039/b904228h

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.

引用

页码：931 / 934

页数：4

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