Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography

被引：2
|
作者：
Myrdal, Paul B. [1 ]
Angersbach, B. Steven [1 ]
Karlage, Kelly [1 ]
Kuehl, Philip J. [1 ]
机构：
[1] Univ Arizona, Coll Pharm, Tucson, AZ 85721 USA
来源：
JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1132卷 / 1-2期
关键词：
lipoxygenase; arachidonic acid metabolism; HETEs; chiral separation; chiralcel OR-RH; HOST-GUEST COMPLEXATION; AMINO-ACID ENANTIOMERS; LUNG TUMORIGENESIS; ESTER SALTS; A/J MICE; RESOLUTION; INHIBITORS; APOPTOSIS; PHASE; FORESTOMACH;
D O I：
10.1016/j.chroma.2006.07.010
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 degrees C. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：315 / 319
页数：5
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