Multimodal two-photon microscopy with electrical tunable lens

被引：0

作者：

Takumi, Noda ^{[1
]}

Quan, Xiangyu ^{[1
]}

Rajput, Sudheesh ^{[1
]}

Matoba, Osamu ^{[1
]}

Awatsuji, Yasuhiro ^{[2
]}

机构：

[1] Kobe Univ, Dept Syst Informat, Nada Ku, 1-1 Rokkodai Cho, Kobe, Hyogo 6578501, Japan

[2] Kyoto Inst Technol, Fac Elect Engn & Elect, Sakyo Ku, Kyoto 6068585, Japan

来源：

BIOMEDICAL IMAGING AND SENSING CONFERENCE 2020 | 2020年 / 11521卷

关键词：

two photon microscopy; high-speed volume imaging; multimodal fluorescence imaging;

D O I：

10.1117/12.2573299

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this research, we have proposed a multimodal fast fluorescent imaging by an electrical tunable lens, and quantitative phase imaging by digital holography. In conventional confocal microscopy, sectioning in z direction is realized either by moving objective lens or sample stage. In order to speed up the imaging, and utilize in the specific applications such as light stimulation system in optogenetics, the sectioning is realized by changing the focal power of an electrical tunable lens. The preliminary experiment using fluorescence beads and its relation between focal power and depth change is shown.

引用

页数：2

共 3 条

[1]

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