Hollow Fiber-Based Liquid Phase Microextraction Combined with High Performance Liquid Chromatography for Determination of Flavonoids in Ginkgo biloba and Urine

被引：0
|
作者：
Lu, Huawei [1 ,2 ]
Wang, Xusheng [1 ]
Liu, Xia [1 ]
Jiang, Shengxiang [1 ]
机构：
[1] Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
来源：
CHEMIA ANALITYCZNA | 2009年 / 54卷 / 03期
关键词：
Hollow fibre; Liquid phase microextraction; Flavonoid; Ginkgo biloba; SAMPLE PREPARATION; EXTRACTION; WATER;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A hollow fibre membrane-based liquid phase microextraction (HF-LPME) method for determination of flavonoids in Ginkgo biloba leaves was developed. Three flavonoids: quercetin, kaempferol, and isorhamnetin were extracted and then analysed by high performance liquid chromatography. The factors affecting HF-LPME, including concentration of donor and acceptor phases, concentration of NaCl, extraction time. and stirring speed were systematically investigated. Under the optimum conditions, calibration plots of reasonable linearity were constructed in the analyte's concentration range of 10-100 mu g L-1. Up to 200-fold enrichment factor for quercetin and up to tens of folds for kaempferol and isorhamnetin were obtained. The method was applied to determine the concentrations of flavonoids in urine and was proven to be an convenient sample preparation technique.
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页码：309 / 319
页数：11
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