Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source

被引：21

作者：

Blandin, Pierre ^{[1
,2
]}

Leveque-Fort, Sandrine ^{[1
,2
]}

Lecart, Sandrine ^{[2
]}

Cossec, Jack C. ^{[3
]}

Potier, Mane-Claude ^{[3
]}

Lenkei, Zsolt ^{[3
]}

Druon, Frederic ^{[2
,4
]}

Georges, Patrick ^{[2
,4
]}

机构：

[1] Univ Paris 11, CNRS, Photophys Mol Lab, F-91405 Orsay, France

[2] Univ Paris 11, Ctr Laser, Ctr Photon Biomed, F-91405 Orsay, France

[3] Ecole Super Phys & Chim Ind Ville Paris, CNRS, Lab Neurobiol & Diversite Cellulaire, F-75013 Paris, France

[4] Univ Paris 11, CNRS, Inst Opt, Lab Charles Fabry, F-91127 Palaiseau, France

来源：

APPLIED OPTICS | 2009年 / 48卷 / 03期

关键词：

AMYLOID PRECURSOR PROTEIN; LIFETIME; ANISOTROPY; IMPACT;

D O I：

10.1364/AO.48.000553

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide-field fluorescence lifetime. imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications. (C) 2009 Optical Society of America

引用

页码：553 / 559

页数：7

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