Studies of partially transforming polyomavirus mutants establish a role for phosphatidylinositol 3-kinase in activation of pp70 S6 kinase

Infection of mouse fibroblasts by wild-type polyomavirus results in increased phosphorylation of ribosomal protein S6 (D. A. Talmage, J. Blenis, and T. L. Benjamin, Mel. Cell. Biol. 8:2309-2315, 1988). Here we identify pp70 S6 kinase (pp70(S6K)) as a target for signal transduction events leading from polyomavirus middle tumor antigen (mT). Two partially transforming virus mutants altered in different mT signalling pathways have been studied to elucidate the pathway leading to S6 phosphorylation. An upstream role for mT-phosphatidylinositol 3-kinase (PI3K) complexes in pp70(S6K) activation is implicated by the failure of 315YF, a mutant unable to promote PI3K binding, to elicit a response. This conclusion is supported by studies using wortmannin, a known inhibitor of PI3K. In contrast, stable interaction of mT with She, a protein thought to be involved upstream of Ras, is dispensable for pp70(S6K) activation. 250YS, a mutant mT which retains a binding site for PI3K but lacks one for She, stimulates pp70(S6K) to wild-type levels. Mutants 315YF and 250YS induce partial transformation of rat fibroblasts with distinct phenotypes, as judged from morphological and growth criteria. Neither mutant induces growth in soft agar, indicating that an increase in S6 phosphorylation, while necessary for cell cycle progression in normal mitogenesis, is not sufficient for anchorage-independent cell growth. In the polyomavirus system, the latter requires integration of signals from mT involving both Shc and PI3K.