Optical coherence tomography and Raman spectroscopy of the ex-vivo retina

被引：39

作者：

Evans, Julia W. ^{[1
,2
,3
]}

Zawadzki, Robert J. ^{[2
]}

Liu, Rui ^{[3
]}

Chan, James W. ^{[1
]}

Lane, Stephen M. ^{[1
,3
]}

Werner, John S. ^{[2
]}

机构：

[1] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA

[2] Univ Calif Davis, Vis Sci & Adv Retinal Imaging Lab, Dept Ophthalmol & Vis Sci, Sacramento, CA 95817 USA

[3] Univ Calif Davis, NSF Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA

来源：

JOURNAL OF BIOPHOTONICS | 2009年 / 2卷 / 6-7期

基金：

美国国家科学基金会;

关键词：

optical coherence tomography; Raman spectroscopy; retinal imaging;

D O I：

10.1002/jbio.200910022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Imaging the structure and correlating it with the biochemical content of the retina holds promise for fundamental research and for clinical applications. Optical coherence tomography (OCT) is commonly used to image the 3D structure of the retina and while the added functionality of biochemical analysis afforded by Raman scattering could provide critical molecular signatures for clinicians and researchers, there are many technical challenges to combine these imaging modalities. We describe an OCT microscope for ex-vivo imaging combined with Raman spectroscopy capable of collecting morphological and molecular information about a sample simultaneously. We present our first results and discuss the challenges to further development of this dual-mode instrument and limitations for future in-vivo retinal imaging. [GRAPHICS] Layout of the combined OCT microscope and Raman spectrometer (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：398 / 406

页数：9

共 18 条

[1]

Beattie JR, 2007, MOL VIS, V13, P1106

[2] Maximum permissible exposures for ocular safety (ANSI 2000), with emphasis on ophthalmic devices [J].

Delori, Francois C. ;

Webb, Robert H. ;

Sliney, David H. .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2007, 24 (05) :1250-1265

[3] Raman spectroscopy in ophthalmology: From experimental tool to applications in vivo [J].

Erckens, RJ ;

Jongsma, FHM ;

Wicksted, JP ;

Hendrikse, F ;

March, WF ;

Motamedi, M .

LASERS IN MEDICAL SCIENCE, 2001, 16 (04) :236-252

[4] MEASUREMENT OF INTRAOCULAR DISTANCES BY BACKSCATTERING SPECTRAL INTERFEROMETRY [J].

FERCHER, AF ;

HITZENBERGER, CK ;

KAMP, G ;

ELZAIAT, SY .

OPTICS COMMUNICATIONS, 1995, 117 (1-2) :43-48

[5] Prospects for in vivo Raman spectroscopy [J].

Hanlon, EB ;

Manoharan, R ;

Koo, TW ;

Shafer, KE ;

Motz, JT ;

Fitzmaurice, M ;

Kramer, JR ;

Itzkan, I ;

Dasari, RR ;

Feld, MS .

PHYSICS IN MEDICINE AND BIOLOGY, 2000, 45 (02) :R1-R59

[6] OPTICAL COHERENCE TOMOGRAPHY [J].

HUANG, D ;

SWANSON, EA ;

LIN, CP ;

SCHUMAN, JS ;

STINSON, WG ;

CHANG, W ;

HEE, MR ;

FLOTTE, T ;

GREGORY, K ;

PULIAFITO, CA ;

FUJIMOTO, JG .

SCIENCE, 1991, 254 (5035) :1178-1181

[7] Performance of fourier domain vs. time domain optical coherence tomography [J].

Leitgeb, R ;

Hitzenberger, CK ;

Fercher, AF .

OPTICS EXPRESS, 2003, 11 (08) :889-894

[8] Real-time assessment of retinal blood flow with ultrafast acquisition by color Doppler Fourier domain optical coherence tomography [J].

Leitgeb, RA ;

Schmetterer, L ;

Drexler, W ;

Fercher, AF ;

Zawadzki, RJ ;

Bajraszewski, T .

OPTICS EXPRESS, 2003, 11 (23) :3116-3121

[9] In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve [J].

Nassif, NA ;

Cense, B ;

Park, BH ;

Pierce, MC ;

Yun, SH ;

Bouma, BE ;

Tearney, GJ ;

Chen, TC ;

de Boer, JF .

OPTICS EXPRESS, 2004, 12 (03) :367-376

[10] Real-time fiber-based multi-functional spectral-domain optical coherence tomography at 1.3 μm [J].

Park, BH ;

Pierce, MC ;

Cense, B ;

Yun, SH ;

Mujat, M ;

Tearney, GJ ;

Bouma, BE ;

de Boer, JF .

OPTICS EXPRESS, 2005, 13 (11) :3931-3944

← 1 2 →