The Development of a Novel Diagnostic Assay That Utilizes a Pseudotyped Vesicular Stomatitis Virus for the Detection of Neutralizing Activity against Crimean-Congo Hemorrhagic Fever Virus

被引：0

作者：

Suda, Yuto ^{[1
,2
]}

Chamberlain, John ^{[3
]}

Dowall, Stuart D. ^{[3
]}

Saijo, Masayuki ^{[2
]}

Horimoto, Taisuke ^{[1
]}

Hewson, Roger ^{[3
]}

Shimojima, Masayuki ^{[2
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Microbiol, Tokyo, Japan

[2] Natl Inst Infect Dis, Dept Virol 1, Special Pathogens Lab, Gakuen 4-7-1, Tokyo 2080011, Japan

[3] Publ Hlth England, Natl Infect Serv, Porton Down, Wilts, England

来源：

JAPANESE JOURNAL OF INFECTIOUS DISEASES | 2018年 / 71卷 / 03期

基金：

日本学术振兴会;

关键词：

ENZYME-LINKED-IMMUNOSORBENT; IMMUNOGLOBULIN-G ANTIBODIES; RECOMBINANT NUCLEOPROTEIN; DUGBE VIRUS; SERA; PATHOGENESIS; EXPRESSION; IGG;

D O I：

10.7883/yoken.JJID.2017.354

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Crimean-Congo hemorrhagic fever virus is a risk group 4 pathogen, which mandates the use of maximum containment facilities, often termed biosafety level 4 or containment level 4 when working with infectious materials. Diagnostic and research work involving live viruses in such laboratories is timeconsuming and inconvenient, resulting in delays. Herein, we show that serum neutralizing activity against the virus can be measured in low-containment laboratories using a pseudotyped virus.

引用

收藏

页码：205 / 208

页数：4

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