Interspecies Chimerism with Mammalian Pluripotent Stem Cells

被引：368

作者：

Wu, Jun ^{[1
]}

Platero-Luengo, Aida ^{[1
]}

Sakurai, Masahiro ^{[1
]}

Sugawara, Atsushi ^{[1
]}

Antonia Gil, Maria ^{[2
]}

Yamauchi, Takayoshi ^{[1
]}

Suzuki, Keiichiro ^{[1
]}

Bogliotti, Yanina Soledad ^{[3
]}

Cuello, Cristina ^{[2
]}

Valencia, Mariana Morales ^{[1
]}

Okumura, Daiji ^{[1
,7
]}

Luo, Jingping ^{[1
]}

Vilarino, Marcela ^{[3
]}

Parrilla, Inmaculada ^{[2
]}

Soto, Delia Alba ^{[3
]}

Martinez, Cristina A. ^{[2
]}

Hishida, Tomoaki ^{[1
]}

Sanchez-Bautista, Sonia ^{[4
]}

Llanos Martinez-Martinez, M. ^{[4
]}

Wang, Huili ^{[3
]}

Nohalez, Alicia ^{[2
]}

Aizawa, Emi ^{[1
]}

Martinez-Redondo, Paloma ^{[1
]}

Ocampo, Alejandro ^{[1
]}

Reddy, Pradeep ^{[1
]}

Roca, Jordi ^{[2
]}

Maga, Elizabeth A. ^{[3
]}

Esteban, Concepcion Rodriguez ^{[1
]}

Berggren, W. Travis ^{[1
]}

Nunez Delicado, Estrella ^{[4
]}

Lajara, Jeronimo ^{[4
]}

Guillen, Isabel ^{[5
]}

Guillen, Pedro ^{[4
,5
]}

Campistol, Josep M. ^{[6
]}

Martinez, Emilio A. ^{[2
]}

Juan Ross, Pablo ^{[3
]}

Belmonte, Juan Carlos Izpisua ^{[1
]}

机构：

[1] Salk Inst Biol Studies, 10010 N Torrey Pines Rd, La Jolla, CA 92037 USA

[2] Univ Murcia, Dept Anim Med & Surg, Campus Espinardo, E-30100 Murcia, Spain

[3] Univ Calif Davis, Dept Anim Sci, One Shields Ave, Davis, CA 95616 USA

[4] Univ Catolica San Antonio Murcia UCAM, Campus Jeronimos,135 Guadalupe, Murcia 30107, Spain

[5] Clin CEMTRO, Clin Ctr Fdn Pedro Guillen, Ave Ventisquero Condesa, Madrid 28035, Spain

[6] Univ Barcelona, Hosp Clin Barcelona IDIBAPS, E-08007 Barcelona, Spain

[7] Kinki Univ, Dept Adv Biosci, Grad Sch Agr, 3327-204 Nakamachi, Nara 6318505, Japan

来源：

CELL | 2017年 / 168卷 / 03期

关键词：

HUMAN SOMATIC-CELLS; IN-VITRO; GENE-EXPRESSION; MULTIPLE GENES; GENERATION; NAIVE; DIFFERENTIATION; LINES; MOUSE; VIVO;

D O I：

10.1016/j.cell.2016.12.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Interspecies blastocyst complementation enables organ-specific enrichment of xenogenic pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in several tissues of gene-edited organogenesis-disabled mice. Besides gaining insights into species evolution, embryogenesis, and human disease, interspecies blastocyst complementation might allow human organ generation in animals whose organ size, anatomy, and physiology are closer to humans. To date, however, whether human PSCs (hPSCs) can contribute to chimera formation in non-rodent species remains unknown. We systematically evaluate the chimeric competency of several types of hPSCs using a more diversified clade of mammals, the ungulates. We find that naive hPSCs robustly engraft in both pig and cattle pre-implantation blastocysts but show limited contribution to post-implantation pig embryos. Instead, an intermediate hPSC type exhibits higher degree of chimerism and is able to generate differentiated progenies in post-implantation pig embryos.

引用

页码：473 / +

页数：29

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