Individual's health is determined by a number of factors stemming from the objective as well as subjective assumptions of life. Health depends on the maturity of the country, health care, and not least from the lifestyle of the individual. Selection of profession and occupation significantly affects health. Conditions and work environment is crucial. The aim of this paper is to identify the determinants of quality of working life, resulting from the work environment that affect physical and mental health of employees in Slovak Republic and its regions. The contribution will focus on the analysis of risk conditions of workplaces in the regions of Slovakia, various factors of working conditions and, on the other hand, number of work injuries, as well as the work incapacity that affect the quality of working life.