Molecular mechanisms in C-Phycocyanin induced apoptosis in human chronic myeloid leukemia cell line-K562

被引：177

作者：

Subhashini, J ^{[1
]}

Mahipal, SVK ^{[1
]}

Reddy, MC ^{[1
]}

Reddy, MM ^{[1
]}

Rachamallu, A ^{[1
]}

Reddanna, P ^{[1
]}

机构：

[1] Univ Hyderabad, Sch Life Sci, Dept Anim Sci, Hyderabad 500046, Andhra Pradesh, India

来源：

BIOCHEMICAL PHARMACOLOGY | 2004年 / 68卷 / 03期

关键词：

C-PC; K562 human chronic myeloid leukemia cell line; apoptosis; PARP; cytochrome C; Bcl-2;

D O I：

10.1016/j.bcp.2004.02.025

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

C-Phycocyanin (C-PC), the major light harvesting biliprotein from Spirulina platensis is of greater importance because of its various biological and pharmacological properties. It is a water soluble, non-toxic fluorescent protein pigment with potent anti-oxidant, anti-inflammatory and anti-cancer properties. In the present study the effect of highly purified C-PC was tested on growth and multiplication of human chronic myeloid leukemia cell line (K562). The results indicate significant decrease (49%) in the proliferation of K562 cells treated with 50 muM C-PC up to 48 h. Further studies involving fluorescence and electron microscope revealed characteristic apoptotic features like cell shrinkage, membrane blebbing and nuclear condensation. Agarose electrophoresis of genomic DNA of cells treated with C-PC showed fragmentation pattern typical for apoptotic cells. Flow cytometric analysis of cells treated with 25 and 50 M C-PC for 48 h showed 14.11 and 20.93% cells in sub-G0/G1 phase, respectively. C-PC treatment of K562 cells also resulted in release of cytochrome c into the cytosol and poly(ADP) ribose polymerase (PAR-P) cleavage. These studies also showed down regulation of anti-apoptotic Bcl-2 but without any changes in pro-apoptotic Bax and thereby tilting the Bcl-2/Bax ratio towards apoptosis. These effects of C-PC appear to be mediated through entry of C-PC into the cytosol by an unknown mechanism. The present study thus demonstrates that C-PC induces apoptosis in K562 cells by cytochrome c release from mitochondria into the cytosol, PARP cleavage and down regulation of Bcl-2. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：453 / 462

页数：10

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