Fast Two-Dimensional NMR Spectroscopy of High Molecular Weight Protein Assemblies

被引：80

作者：

Amero, Carlos ^{[1
]}

Schanda, Paul ^{[1
,2
]}

Dura, M. Asuncion ^{[1
]}

Ayala, Isabel ^{[1
]}

Marion, Dominique ^{[1
]}

Franzetti, Bruno ^{[1
]}

Brutscher, Bernhard ^{[1
]}

Boisbouvier, Jerome ^{[1
]}

机构：

[1] UJF, CEA, CNRS, Inst Biol Struct Jean Pierre Ebel, F-38027 Grenoble, France

[2] ETH, Phys Chem Lab, CH-8093 Zurich, Switzerland

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 10期

关键词：

MULTIDIMENSIONAL NMR; METHYL; SECONDS; SPECTRA; ALANINE; COMPLEX;

D O I：

10.1021/ja809880p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl. H-1-C-13 correlation spectra of protein assemblies of several hundreds of kDa in a few seconds. The SOFAST-methyl-TROSY-based experiment offers completely new opportunities for the study of structural and dynamic changes occurring in molecular nanomachines while they perform their biological function in vitro.

引用

页码：3448 / +

页数：3

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