Fast Two-Dimensional NMR Spectroscopy of High Molecular Weight Protein Assemblies

被引：80
|
作者：
Amero, Carlos [1 ]
Schanda, Paul [1 ,2 ]
Dura, M. Asuncion [1 ]
Ayala, Isabel [1 ]
Marion, Dominique [1 ]
Franzetti, Bruno [1 ]
Brutscher, Bernhard [1 ]
Boisbouvier, Jerome [1 ]
机构：
[1] UJF, CEA, CNRS, Inst Biol Struct Jean Pierre Ebel, F-38027 Grenoble, France
[2] ETH, Phys Chem Lab, CH-8093 Zurich, Switzerland
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 10期
关键词：
MULTIDIMENSIONAL NMR; METHYL; SECONDS; SPECTRA; ALANINE; COMPLEX;
D O I：
10.1021/ja809880p
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl. H-1-C-13 correlation spectra of protein assemblies of several hundreds of kDa in a few seconds. The SOFAST-methyl-TROSY-based experiment offers completely new opportunities for the study of structural and dynamic changes occurring in molecular nanomachines while they perform their biological function in vitro.
引用
收藏
页码：3448 / +
页数：3
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