Development of a simple functional marker for fragrance in rice and its validation in Indian Basmati and non-Basmati fragrant rice varieties

被引：49

作者：

Sakthivel, K. ^{[1
]}

Rani, N. Shobha ^{[1
]}

Pandey, Manish K. ^{[1
]}

Sivaranjani, A. K. P. ^{[1
]}

Neeraja, C. N. ^{[1
]}

Balachandran, S. M. ^{[1
]}

Madhav, M. Sheshu ^{[1
]}

Viraktamath, B. C. ^{[1
]}

Prasad, G. S. V. ^{[1
]}

Sundaram, R. M. ^{[1
]}

机构：

[1] Directorate Rice Res, Crop Improvement Sect, Hyderabad 500030, Andhra Pradesh, India

来源：

MOLECULAR BREEDING | 2009年 / 24卷 / 02期

关键词：

Oryza sativa; Rice; Fragrance; Aroma; badh2; Functional marker; GENE; AROMA;

D O I：

10.1007/s11032-009-9283-x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Fragrance development in rice has been reported due to a 8-bp deletion in the exon 7 of badh2 gene located on Chromosome 8S. Multiplex markers targeting the functional InDel polymorphism was earlier reported for genotyping fragrance trait, but the marker was observed to be inconsistent and difficult to use. We have developed a simple, co-dominant, functional marker for fragrance trait, which can be resolved in an agarose gel and validated in Basmati and non-Basmati aromatic rice varieties and in a mapping population segregated for fragrance trait. The marker targets the InDel polymorphism in badh2 gene and amplifies 95 and 103 bp fragments in fragrant and non-fragrant genotypes, respectively. The newly developed marker was highly efficient in discriminating all fragrant and non-fragrant genotypes and showed perfect co-segregation with the trait of fragrance in the mapping population. We recommend the use of this simple, low-cost marker in routine genotyping for fragrance trait in large scale breeding materials and germplasm.

引用

页码：185 / 190

页数：6

共 19 条

[1] RFLP TAGGING OF A GENE FOR AROMA IN RICE [J].

AHN, SN ;

BOLLICH, CN ;

TANKSLEY, SD .

THEORETICAL AND APPLIED GENETICS, 1992, 84 (7-8) :825-828

[2] Mapping of quantitative trait loci for basmati quality traits in rice (Oryza sativa L.) [J].

Amarawathi, Yellari ;

Singh, Rakesh ;

Singh, Ashok K. ;

Singh, Vijai P. ;

Mohapatra, Trilochan ;

Sharma, Tilak R. ;

Singh, Nagendra K. .

MOLECULAR BREEDING, 2008, 21 (01) :49-65

[3] INHERITANCE OF SCENT IN AMERICAN LONG GRAIN RICE [J].

BERNER, DK ;

HOFF, BJ .

CROP SCIENCE, 1986, 26 (05) :876-878

[4] A perfect marker for fragrance genotyping in rice [J].

Bradbury, LMT ;

Henry, RJ ;

Jin, QS ;

Reinke, RF ;

Waters, DLE .

MOLECULAR BREEDING, 2005, 16 (04) :279-283

[5] The gene for fragrance in rice [J].

Bradbury, LMT ;

Fitzgerald, TL ;

Henry, RJ ;

Jin, QS ;

Waters, DLE .

PLANT BIOTECHNOLOGY JOURNAL, 2005, 3 (03) :363-370

[6]

BUTTERY RG, 1982, CHEM IND-LONDON, P958

[7]

Dellaporta S.L., 1983, Plant Mol Biol Report, V1, P19, DOI DOI 10.1007/BF02712670

[8] Is there a second fragrance gene in rice? [J].

Fitzgerald, Melissa A. ;

Hamilton, N. Ruaraidh Sackville ;

Calingacion, Mariafe N. ;

Verhoeven, Harrie A. ;

Butardo, Vito M. .

PLANT BIOTECHNOLOGY JOURNAL, 2008, 6 (04) :416-423

[9]

*IND AGR RES I, 1980, IARI TECH RES B, V30, P46

[10]

KUO SM, 2005, P 5 INT RIC GEN S IR, P166

← 1 2 →