Chemokines expression during Leptospira interrogans serovar Copenhageni infection in resistant BALB/c and susceptible C3H/HeJ mice

被引：28
作者：
da Silva, Josefa B. [1 ,2 ]
Ramos, Tatiane M. V. [3 ]
de Franco, Marcelo [4 ]
Paiva, Delhi [5 ]
Ho, Paulo Lee [1 ,2 ]
Martins, Elizabeth A. L. [1 ,2 ]
Pereira, Martha M. [3 ]
机构：
[1] Inst Butantan, Ctr Biotecnol, BR-05303900 Sao Paulo, Brazil
[2] Univ Sao Paulo, Interunidades Biotecnol, Inst Ciencias Biomed, BR-09500900 Sao Paulo, Brazil
[3] Fiocruz MS, Inst Oswaldo Cruz, WHO Collaborating Ctr Leptospirosis, BR-21045900 Rio De Janeiro, Brazil
[4] Inst Butantan, Lab Imunogenet, BR-05303900 Sao Paulo, Brazil
[5] Univ Sao Paulo, Inst Matemat & Estatist, Sao Paulo, Brazil
来源：
MICROBIAL PATHOGENESIS | 2009年 / 47卷 / 02期
基金：
巴西圣保罗研究基金会;
关键词：
Leptospira; Chemokines; Mice C3H/HeJ; RENAL-DISEASE; T-CELLS; PROTEIN-1; ICTEROHAEMORRHAGIAE; IDENTIFICATION; MONOCYTES; INSIGHTS; CD4(+); LUNG; PCR;
D O I：
10.1016/j.micpath.2009.05.002
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The role of innate immune responses in protection against leptospirosis remains unclear. We examined the expression of the chemokines CCL2/JE (MCP-1), CCL3/MIP-1 alpha (MIP-1 alpha) and CXCL1/KC (IL-8) regarding resistance and susceptibility to leptospirosis in experimental mice models BALB/c and C3H/HeJ, respectively. A virulent strain of Leptospira interrogans serovar Copenhageni was used in this study. Twenty-five animals of each mouse strain of C3H/HeJ and BALB/c, were infected intraperitoneally with 106 cells. Five un-infected animals of each strain were kept as control. Mortality of C3H/HeJ mouse was observed while BALB/c mice were asymptomatic. The presence of leptospire DNA in tissues of infected animals was demonstrated by PCR. Chemokines were measured in serum, spleen, liver, kidney and lung of both strains of animals using immunoenzymatic assay (ELISA). Elevations in the levels of chemokines MCP-1 and IL-8 occurred in all organs and sera of C3H/HeJ and BALB/c infected mice. The levels of MIP-1 alpha were lower when compared to MCP-1 and IL-8 in all analyzed organs, with a slight increase in liver and kidney. Our results indicate that the expression of inflammatory mediators can vary greatly, depending on the tissue and mouse strains. It is possible that the resistance to Leptospira can be partially correlated to the increase of MIP-1 alpha observed in BALB/c mice, while an increasing and a sustained expression of MCP-1 and IL-8 in the lungs of C3H/HeJ mice can be correlated to the severity and progression of leptospirosis. (C) 2009 Elsevier Ltd. All rights reserved.
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页码：87 / 93
页数：7
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