RT-PCR based diagnosis revealed importance of human group B rotavirus infection in childhood diarrhoea

被引:24
作者
Barman, P.
Ghosh, S.
Samajdar, S.
Mitra, U.
Dutta, P.
Bhattacharya, S. K.
Krishnan, T.
Kobayashi, N.
Naik, T. N.
机构
[1] Natl Inst Cholera & Enter Dis, Div Virol, Kolkata 700010, W Bengal, India
[2] Natl Inst Cholera & Enter Dis, Div Clin Med, Kolkata 700010, W Bengal, India
[3] Sapporo Med Univ, Sch Med, Dept Hyg, Sapporo, Hokkaido 060, Japan
关键词
human group B rotavirus; children; PAGE; RT-PCR; nonisotopic dot-blot hybridization; sequence analysis;
D O I
10.1016/j.jcv.2006.02.009
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Human group B rotavirus was first identified as causative agent of a large outbreak of severe gastroenteritis affecting more than I million people, predominantly adults in China in 1982-1983. In spite of serological evidences for the presence of group B rotavirus in many countries of the world, the virus has been detected only from China, India and Bangladesh, where most of the cases were from adults. Objectives: To ascertain the role of group B rotavirus as an aetiological agent of diarrhoea among children in Kolkata, India. Study design: An active surveillance was conducted for rotavirus infection in children in a leading referral paediatric hospital and a few samples were also collected from adults of another hospital in Kolkata, India over a period of 3 years (2002-2004). After primary screening of rotaviruses by RNA electrophoresis in polyacrylamide get, 200 of 412 samples negative by PAGE were screened by reverse transcription polymerase chain reaction for group B rotaviruses. The group B rotavirus positives samples were also confirmed by dot-blot hybridization. Result: During the study period, we detected 37 (18.5%) sporadic cases of human group B rotavirus infection in children below 3 years of age of which 15 (7.5%) showed mixed infection with group A rotaviruses by RT-PCR. In dot-blot hybridization studies the RNA of all rotavirus positive samples hybridized with the nonisotopic psoralen-biotin labeled total RNA probe generated from a human group B rotavirus CAL-1 strain confirming the samples as group B rotaviruses. Conclusion: The shift in age preference of group B rotavirus infection from adult to children and mixed infection of group B and group A rotaviruses reveals the importance of group B rotavirus as an etiological agent of childhood diarrhoea. Therefore, future vaccination strategy should include both group A and B rotaviruses to control rotavirus diarrhoea. (c) 2006 Elsevier B.V. All rights reserved.
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页码:222 / 227
页数:6
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