An electron transfer competent structural ensemble of membrane-bound cytochrome P450 1A1 and cytochrome P450 oxidoreductase

被引:26
作者
Mukherjee, Goutam [1 ,2 ,3 ]
Nandekar, Prajwal P. [1 ,2 ,4 ]
Wade, Rebecca C. [1 ,2 ,3 ]
机构
[1] Heidelberg Inst Theoret Studies HITS, Mol & Cellular Modeling Grp, Schloss Wolfsbrunnenweg 35, D-69118 Heidelberg, Germany
[2] Heidelberg Univ, DKFZ ZMBH Alliance, Zentrum Mol Biol, INF 282, D-69120 Heidelberg, Germany
[3] Heidelberg Univ, Interdisciplinary Ctr Sci Comp IWR, INF 368, D-69120 Heidelberg, Germany
[4] Schrodinger Inc, 147,3rd Floor,Jawaharlal Nehru Main Rd,5th Stage, Bengaluru 560098, India
关键词
MOLECULAR-DYNAMICS; TRANSFER PATHWAY; FUSED ENZYME; PROTEIN; REDUCTASE; P450; ASSOCIATION; COMPLEX; CYP1A1; DOMAIN;
D O I
10.1038/s42003-020-01568-y
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cytochrome P450 (CYP) heme monooxygenases require two electrons for their catalytic cycle. For mammalian microsomal CYPs, key enzymes for xenobiotic metabolism and steroidogenesis and important drug targets and biocatalysts, the electrons are transferred by NADPH-cytochrome P450 oxidoreductase (CPR). No structure of a mammalian CYP-CPR complex has been solved experimentally, hindering understanding of the determinants of electron transfer (ET), which is often rate-limiting for CYP reactions. Here, we investigated the interactions between membrane-bound CYP 1A1, an antitumor drug target, and CPR by a multiresolution computational approach. We find that upon binding to CPR, the CYP 1A1 catalytic domain becomes less embedded in the membrane and reorients, indicating that CPR may affect ligand passage to the CYP active site. Despite the constraints imposed by membrane binding, we identify several arrangements of CPR around CYP 1A1 that are compatible with ET. In the complexes, the interactions of the CPR FMN domain with the proximal side of CYP 1A1 are supplemented by more transient interactions of the CPR NADP domain with the distal side of CYP 1A1. Computed ET rates and pathways agree well with available experimental data and suggest why the CYP-CPR ET rates are low compared to those of soluble bacterial CYPs. Mukherjee, Nandekar and Wade investigate the structural arrangement of the complex between membrane-bound cytochrome P450 1A1 and NADPH-cytochrome P450 reductase. They find that upon binding to the reductase, the catalytic domain of cytochrome P450 1A1 reorients subject to the constraints of membrane binding, potentially explaining why the electron transfer rates between the proteins are low when compared to those of soluble bacterial cytochrome P450s.
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页数:13
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