Induction of human cytotoxic T lymphocytes by artificial antigen-presenting cells

被引：148

作者：

Latouche, JB

Sadelain, M

机构：

[1] Mem Sloan Kettering Canc Ctr, Dept Human Genet, New York, NY 10021 USA

[2] Mem Sloan Kettering Canc Ctr, Program Immunol, New York, NY 10021 USA

来源：

NATURE BIOTECHNOLOGY | 2000年 / 18卷 / 04期

关键词：

adoptive cell therapy; CD8(+) T cell; costimulation; dendritic cells; immunotherapy; retrovirus-mediated gene transfer;

D O I：

10.1038/74455

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic approach for a number of diseases. To overcome the difficulty in generating specific CTLs, we established stable artificial antigen-presenting cells (AAPCs) that can be used to stimulate T cells of any patient of a given human leukocyte antigen (HLA) type. Mouse fibroblasts were retrovirally transduced with a single HLA-peptide complex along with the human accessory molecules B7.1, ICAM-1, and LFA-3. These AAPCs consistently elicit strong stimulation and expansion of HLA-restricted CTLs. Owing to the high efficiency of retrovirus-mediated gene transfer, stable AAPCs can be readily engineered for any HLA molecule and any specific peptide.

引用

页码：405 / 409

页数：5

共 46 条

[1] ENDOGENOUSLY SYNTHESIZED PEPTIDE WITH AN ENDOPLASMIC-RETICULUM SIGNAL SEQUENCE SENSITIZES ANTIGEN PROCESSING MUTANT-CELLS TO CLASS-I-RESTRICTED CELL-MEDIATED LYSIS [J].