Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine

被引:643
作者
Cheng, Ji-Xin [1 ,2 ]
Xie, X. Sunney [3 ]
机构
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
[3] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA
关键词
STIMULATED RAMAN-SCATTERING; IN-VIVO; CARS MICROSCOPY; LABEL-FREE; LIVE CELLS; PHOTOACOUSTIC TOMOGRAPHY; DEEP-TISSUE; SPECTRAL-ANALYSIS; PHOTON MIGRATION; FIBEROPTIC ARRAY;
D O I
10.1126/science.aaa8870
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Vibrational spectroscopy has been extensively applied to the study of molecules in gas phase, in condensed phase, and at interfaces. The transition from spectroscopy to spectroscopic imaging of living systems, which allows the spectrum of biomolecules to act as natural contrast, is opening new opportunities to reveal cellular machinery and to enable molecule-based diagnosis. Such a transition, however, involves more than a simple combination of spectrometry and microscopy. We review recent efforts that have pushed the boundary of the vibrational spectroscopic imaging field in terms of spectral acquisition speed, detection sensitivity, spatial resolution, and imaging depth. We further highlight recent applications in functional analysis of single cells and in label-free detection of diseases.
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页数:84
相关论文
共 148 条
[1]   Spectroscopic photoacoustic imaging of lipid-rich plaques in the human aorta in the 740 to 1400 nm wavelength range [J].
Allen, Thomas J. ;
Hall, Andrew ;
Dhillon, Amar P. ;
Owen, James S. ;
Beard, Paul C. .
JOURNAL OF BIOMEDICAL OPTICS, 2012, 17 (06)
[2]   Stimulated Raman scattering microscopy by spectral focusing and fiber-generated soliton as Stokes pulse [J].
Andresen, Esben Ravn ;
Berto, Pascal ;
Rigneault, Herve .
OPTICS LETTERS, 2011, 36 (13) :2387-2389
[3]   COHERENT ANTI-STOKES RAMAN-SPECTROSCOPY [J].
BEGLEY, RF ;
HARVEY, AB ;
BYER, RL .
APPLIED PHYSICS LETTERS, 1974, 25 (07) :387-390
[4]   Stimulated Raman scattering using a single femtosecond oscillator with flexibility for imaging and spectral applications [J].
Beier, Hope T. ;
Noojin, Gary D. ;
Rockwell, Benjamin A. .
OPTICS EXPRESS, 2011, 19 (20) :18885-18892
[5]   In vivo evaluation of demyelination and remyelination in a nerve crush injury model [J].
Belanger, E. ;
Henry, F. P. ;
Vallee, R. ;
Randolph, M. A. ;
Kochevar, I. E. ;
Winograd, J. M. ;
Lin, C. P. ;
Cote, D. .
BIOMEDICAL OPTICS EXPRESS, 2011, 2 (09) :2698-2708
[6]  
Bell A. G., 1880, AM J SCI, V3, p305 324
[7]   Quantitative detection of C-deuterated drugs by CARS microscopy and Raman microspectroscopy [J].
Bergner, Gero ;
Albert, Christian R. ;
Schiller, Markus ;
Bringmann, Gerhard ;
Schirmeister, Tanja ;
Dietzek, Benjamin ;
Niebling, Stephan ;
Schluecker, Sebastian ;
Popp, Juergen .
ANALYST, 2011, 136 (18) :3686-3693
[8]   Infrared Spectroscopic Imaging: The Next Generation [J].
Bhargava, Rohit .
APPLIED SPECTROSCOPY, 2012, 66 (10) :1091-1120
[9]   Decomposition of in vivo spatially offset Raman spectroscopy data using multivariate analysis techniques [J].
Buckley, Kevin ;
Kerns, Jemma G. ;
Parker, Anthony W. ;
Goodship, Allen E. ;
Matousek, Pavel .
JOURNAL OF RAMAN SPECTROSCOPY, 2014, 45 (02) :188-192
[10]  
Camp CH, 2014, NAT PHOTONICS, V8, P627, DOI [10.1038/NPHOTON.2014.145, 10.1038/nphoton.2014.145]